Calcification microstructure reflects breast tissue microenvironment

Show simple item record Gosling, Sarah Scott, Robert Greenwood, Charlene Bouzy, Pascaline Nallala, Jayakrupakar Lyburn, Iain Douglas Stone, Nicholas Rogers, Keith 2020-01-17T16:33:27Z 2020-01-17T16:33:27Z 2019-12-05
dc.identifier.citation Gosling S, Scott R, Greenwood C, et al., (2019) Calcification microstructure reflects breast tissue microenvironment. Journal of Mammary Gland Biology and Neoplasia, Volume 24, Issue 4, December 2019, pp. 333-342 en_UK
dc.identifier.issn 1083-3021
dc.description.abstract Microcalcifications are important diagnostic indicators of disease in breast tissue. Tissue microenvironments differ in many aspects between normal and cancerous cells, notably extracellular pH and glycolytic respiration. Hydroxyapatite microcalcification microstructure is also found to differ between tissue pathologies, including differential ion substitutions and the presence of additional crystallographic phases. Distinguishing between tissue pathologies at an early stage is essential to improve patient experience and diagnostic accuracy, leading to better disease outcome. This study explores the hypothesis that microenvironment features may become immortalised within calcification crystallite characteristics thus becoming indicators of tissue pathology. In total, 55 breast calcifications incorporating 3 tissue pathologies (benign – B2, ductal carcinoma in-situ - B5a and invasive malignancy - B5b) from archive formalin-fixed paraffin-embedded core needle breast biopsies were analysed using X-ray diffraction. Crystallite size and strain were determined from 548 diffractograms using Williamson-Hall analysis. There was an increased crystallinity of hydroxyapatite with tissue malignancy compared to benign tissue. Coherence length was significantly correlated with pathology grade in all basis crystallographic directions (P < 0.01), with a greater difference between benign and in situ disease compared to in-situ disease and invasive malignancy. Crystallite size and non-uniform strain contributed to peak broadening in all three pathologies. Furthermore, crystallite size and non-uniform strain normal to the basal planes increased significantly with malignancy (P < 0.05). Our findings support the view that tissue microenvironments can influence differing formation mechanisms of hydroxyapatite through acidic precursors, leading to differential substitution of carbonate into the hydroxide and phosphate sites, causing significant changes in crystallite size and non-uniform strain. en_UK
dc.language.iso en en_UK
dc.publisher Springer en_UK
dc.rights Attribution 4.0 International *
dc.rights.uri *
dc.subject Hydroxyapatite en_UK
dc.subject Carbonate en_UK
dc.subject Breast Cancer en_UK
dc.subject Calcification en_UK
dc.subject X-ray diffraction en_UK
dc.title Calcification microstructure reflects breast tissue microenvironment en_UK
dc.type Article en_UK

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