Pressure tolerance of Artemia cysts compressed in water medium

Show simple item record Hazael, Rachael Matsuda, Shinsuke Mori, Yoshihisa Fitzmaurice, Brianna Appleby-Thomas, Gareth J. Painter, Jonathan Meersman, Filip Richaud, Myriam Galas, Simon Saini, Naurang L. Ono, Fumihisa McMillan, Paul F. 2019-04-04T14:33:39Z 2019-04-04T14:33:39Z 2019-02-04
dc.identifier.citation Hazael R, Matsuda S, Mori Y, et al., Pressure tolerance of Artemia cysts compressed in water medium. High Pressure Research Article, Volume 39, Issue 2, 10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018) , pp. 293-300 en_UK
dc.identifier.issn 0895-7959
dc.description.abstract The high pressure tolerance of cysts of Artemia salina was investigated up to several GPa in water. No survival was observed after exposure to 1.0 GPa for 15 min. After exposure to 2.0 GPa for the same time duration, the hatching rate had recovered to 33%, but decreased to 8% following compression at 7.5 GPa. This contrasts with results using Fluorinert™ as the pressure-transmitting medium where 80–88% recovery was observed. The lower survival rate in water is accompanied by swelling of the eggs, indicating that liquid H2O close to the ice-VI crystallization pressure penetrated inside the eggs. This pressure exceeds the stability limit for proteins and other key biomolecules components within the embryos that could not be resuscitated. Rehydration takes several minutes and so was not completed for all samples compressed to higher pressures, prior to ice-VI formation, resulting in renewed survival. However H2O penetration inside the shell resulted in increased mortality en_UK
dc.language.iso en en_UK
dc.publisher Taylor and Francis en_UK
dc.relation.ispartofseries ;GHPR 1575378
dc.rights Attribution-NonCommercial 4.0 International *
dc.rights.uri *
dc.subject Artemia salina en_UK
dc.subject high pressure tolerance en_UK
dc.subject hatchability en_UK
dc.subject osmotic pressure en_UK
dc.subject ice VI phase en_UK
dc.title Pressure tolerance of Artemia cysts compressed in water medium en_UK
dc.type Article en_UK

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