Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection

Show simple item record Ashley, Jon D’Aurelio, Roberta Piekarska, Monika Temblay, Jeff Pleasants, Mike Trinh, Linda Rodgers, Thomas L. Tothill, Ibtisam E. 2019-01-09T15:54:25Z 2019-01-09T15:54:25Z 2018-03-27
dc.identifier.citation Jon Ashley, Roberta D’Aurelio, Monika Piekarska, et al., Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection. Biosensors, 2018, Volume 8, Issue 2, Article number 32 en_UK
dc.identifier.issn 2079-6374
dc.description.abstract A sensitive and label-free surface plasmon resonance (SPR) based sensor was developed in this work for the detection of milk allergens. β-lactoglobulin (BLG) protein was used as the biomarker for cow milk detection. This is to be used directly in final rinse samples of cleaning in-place (CIP) systems of food manufacturers. The affinity assay was optimised and characterised before a standard curve was performed in pure buffer conditions, giving a detection limit of 0.164 µg mL−1 as a direct binding assay. The detection limit can be further enhanced through the use of a sandwich assay and amplification with nanomaterials. However, this was not required here, as the detection limit achieved exceeded the required allergen detection levels of 2 µg mL−1 for β-lactoglobulin. The binding affinities of the polyclonal antibody for BLG, expressed by the dissociation constant (KD), were equal to 2.59 × 10−9 M. The developed SPR-based sensor offers several advantages in terms of label-free detection, real-time measurements, potential on-line system and superior sensitivity when compared to ELISA-based techniques. The method is novel for this application and could be applied to wider food allergen risk management decision(s) in food manufacturing. en_UK
dc.language.iso en en_UK
dc.publisher MDPI en_UK
dc.rights Attribution 4.0 International *
dc.rights.uri *
dc.subject Milk protein en_UK
dc.subject Surface plasmon resonance (SPR) en_UK
dc.subject Allergen en_UK
dc.subject Biosensor en_UK
dc.subject β-lactoglobulin (BLG) en_UK
dc.title Development of a β-Lactoglobulin sensor based on SPR for milk allergens detection en_UK
dc.type Article en_UK

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