Water dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scattering

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dc.contributor.author Foglia, Fabrizia
dc.contributor.author Hazael, Rachael
dc.contributor.author Simeoni, Giovanna G.
dc.contributor.author Appavou, Marie-Sousai
dc.contributor.author Moulin, Martine
dc.contributor.author Haertlein, Michael
dc.contributor.author Forsyth, V. Trevor
dc.contributor.author Seydel, Tilo
dc.contributor.author Daniel, Isabelle
dc.contributor.author Meersman, Filip
dc.contributor.author McMillan, Paul F.
dc.date.accessioned 2018-02-16T15:35:45Z
dc.date.available 2018-02-16T15:35:45Z
dc.date.issued 2016-01-07
dc.identifier.citation Foglia F, Hazael R, Simeoni GG, Appavou M-S, Moulin M, Haertlein M, Forsyth VT, Seydel T, Daniel I, Meersman F, McMillan PF, Water dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scattering, Scientific Reports, Vol. 6, 2016, Article number 18862 en_UK
dc.identifier.issn 2045-2322
dc.identifier.uri http://dx.doi.org/10.1038/srep18862
dc.identifier.uri http://dspace.lib.cranfield.ac.uk/handle/1826/13008
dc.description.abstract Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico- to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth’s deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7–1.1 Å−1 corresponding to real space dimensions of 6–9 Å. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures. en_UK
dc.language.iso en en_UK
dc.publisher Nature Publishing Group en_UK
dc.rights Attribution 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.title Water dynamics in Shewanella oneidensis at ambient and high pressure using quasi-elastic neutron scattering en_UK
dc.type Article en_UK
dc.identifier.cris 19469733

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