Highly sensitive and selective biosensor based on graphene oxide coated long period grating

Abstract

We propose an optical fiber immunosensor based on graphene oxide coated dual-peak long period grating (GO-dLPG), in which GO-IgG linking layer is used for rapid immunoassays. The binding interaction between antibody and antigen produced a detectable optical signal in terms of grating resonant wavelength shift, which was proportional to the analyte concentration. By deposition of GO overlay, the bulk RI sensitivity of dLPG was enhanced around 150%. The GO-coated dLPG was biofunctionalized by the immobilization of IgG to generate the biosensor. The IgG-bound GO-dLPG was used to detect the anti-IgG and anti-PSA, respectively, demonstrating high sensitivity and selectivity. The GO-dLPG biosensor can be further developed as a biosensing platform with advantages of label-free, real-time and low limit of detection.