Surface plasmon pesonance immunosensor for the detection of campylobacter jejuni

Show simple item record Masdor, Noor Azlina Altintas, Zeynep Tothill, Ibtisam E. 2017-05-17T11:05:19Z 2017-05-17T11:05:19Z 2017-05-16
dc.identifier.citation Masdor NA, Altintas Z, Tothill IE. (2017) Surface plasmon pesonance immunosensor for the detection of campylobacter jejuni. Chemosensors, Volume 5, Issue 2, May 2017, Article number 16 en_UK
dc.identifier.issn 2227-9040
dc.description.abstract Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 106 CFU·mL−1. The sensitivity of the assay was further enhanced to 4 × 104 CFU·mL−1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (106–107 CFU·mL−1). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni. en_UK
dc.language.iso en en_UK
dc.publisher MDPI en_UK
dc.rights Attribution 4.0 International
dc.subject Campylobacter jejuni en_UK
dc.subject campylobacteriosis en_UK
dc.subject Surface Plasmon Resonance (SPR) en_UK
dc.subject immunosensor en_UK
dc.subject sandwich assay en_UK
dc.title Surface plasmon pesonance immunosensor for the detection of campylobacter jejuni en_UK
dc.type Article en_UK

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search CERES


My Account