Modelling the effect of combined antimicrobials: A base model for multiple-hurdles

Show simple item record Anastasiadi, Maria Lambert, R. J. 2017-05-02T08:32:50Z 2017-05-02T08:32:50Z 2017-04-14
dc.identifier.citation Anastasiadi M, Lambert RJ, Modelling the effect of combined antimicrobials: A base model for multiple-hurdles, International Journal of Food Microbiology, Volume 252, 3 July 2017, Pages 10 – 17. en_UK
dc.identifier.issn 0168-1605
dc.description.abstract Combining antimicrobials to reduce microbial growth and to combat the potential impact of antimicrobial resistance is an important subject both in foods and in pharmaceutics. Modelling of combined treatments designed to reduce or eliminate microbial contamination in foods (microbiological predictive modelling) has become commonplace. Two main reference models are used to analyse mixtures: the Bliss Independence and the Loewe reference models (LRM). By using optical density to analyse the growth of Aeromonas hydrophila, Cronobacter sakazakii and Escherichia coli in combined NaCl/NaCl (a mock combination experiment) and combined NaCl/KCl experiments, previous models for combined antimicrobials in foods, based on the Bliss approach, were shown to be inconsistent and that models based on the LRM more applicable. The LRM was shown, however, to be valid only in the specific cases where the concentration exponents of all components in a mixture were identical. This is assured for a mock combination experiment but not for a true mixture. This, essentially, invalidates the LRM as a general reference model. A new model, based on the LRM but allowing for mixed exponents, was used to analyse the combined inhibition data, and concluded that the NaCl/KCl system gave the additive effect expected from literature studies. This study suggests the need to revise current models used to analyse combined effects. en_UK
dc.language.iso en en_UK
dc.publisher Elsevier en_UK
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International
dc.subject Combination index en_UK
dc.subject Synergism en_UK
dc.subject Modelling en_UK
dc.subject Gamma model en_UK
dc.title Modelling the effect of combined antimicrobials: A base model for multiple-hurdles en_UK
dc.type Article en_UK

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