Fabrication and optimisation of a fused filament 3D-printed microfluidic platform

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dc.contributor.author Tothill, Alexander M.
dc.contributor.author Partridge, Matthew
dc.contributor.author James, Stephen W.
dc.contributor.author Tatam, Ralph P.
dc.date.accessioned 2017-02-15T15:16:37Z
dc.date.available 2017-02-15T15:16:37Z
dc.date.issued 2017-02-15
dc.identifier.citation Tothill A, Partridge M, James SW, Tatam R, Fabrication and optimisation of a fused filament 3D-printed microfluidic platform, Journal of Micromechanics and Microengineering, Volume 27, Issue 3, article number 035018 en_UK
dc.identifier.issn 0960-1317
dc.identifier.uri http://dx.doi.org/10.1088/1361-6439/aa5ae3
dc.identifier.uri https://dspace.lib.cranfield.ac.uk/handle/1826/11449
dc.description.abstract A 3D-printed microfluidic device was designed and manufactured using a low cost ($2000) consumer grade fusion deposition modelling (FDM) 3D printer. FDM printers are not typically used, or are capable, of producing the fine detailed structures required for microfluidic fabrication. However, in this work, the optical transparency of the device was improved through manufacture optimisation to such a point that optical colorimetric assays can be performed in a 50 µl device. A colorimetric enzymatic cascade assay was optimised using glucose oxidase and horseradish peroxidase for the oxidative coupling of aminoantipyrine and chromotropic acid to produce a blue quinoneimine dye with a broad absorbance peaking at 590 nm for the quantification of glucose in solution. For comparison the assay was run in standard 96 well plates with a commercial plate reader. The results show the accurate and reproducible quantification of 0–10 mM glucose solution using a 3D-printed microfluidic optical device with performance comparable to that of a plate reader assay. en_UK
dc.language.iso en en_UK
dc.publisher IOP Publishing: Hybrid Open Access en_UK
dc.rights Attribution 3.0 International
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject 3D-printing en_UK
dc.subject microfluidics en_UK
dc.subject devices en_UK
dc.subject glucose en_UK
dc.subject enzymatic en_UK
dc.title Fabrication and optimisation of a fused filament 3D-printed microfluidic platform en_UK
dc.type Article en_UK

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