Staff publications - Cranfield Health
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Browsing Staff publications - Cranfield Health by Type "Conference paper"
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Item Open Access Assessment of risk from inhalation exposure to benzene: a case study(2007-05-01T00:00:00Z) Crump, Derrick; Brown, Veronica M.; Carson, Anthony; Harrison, PaulWhen assessing the possible risks to human health associated with contaminated land, one of the potential pathways of exposure to be considered is the release of chemical vapours that might be inhaled by people via ambient and indoor air. Concentrations in indoor air can be relatively high because of the occurrence of preferential pathways for soil gas movement into buildings and because of a build up of concentrations in the enclosed space (Crump, 2004).Item Open Access Biochemical and textural ripeness assessment of avocado fruit from different origins(International Society for Horticultural Science (ISHS), 2010-10-06T00:00:00Z) Landahl, Sandra; Meyer, Marjolaine D.; Terry, Leon A.Stage of ripeness and quality of avocado is notoriously difficult to assess by conventional methods. Texture is a very important determinant of avocado fruit quality and can change radically during storage. The difficulties in determining avocado quality are related, in part, to the spatial heterogeneity of fruit characteristics during ripening. The aim of this study was to assess the discriminatory capabilities of physiochemical properties to determine ripeness of imported 'Hass' avocado fruit. Fruit were stored at 12°C and sampled at regular intervals. Using a previously unreported method, the texture of different slices from individual fruit was measured during ripening. Maximum load, elasticity and viscosity of fruit tissue were measured using a universal testing machine fitted with a 500N or 5N load cell. The same tissue slice was then further processed prior to subsequent quantification of non-structural carbohydrates (NSCs) and fatty acid methyl esters using standard high performance liquid chromatography coupled to evaporative light scattering detection and gas chromatography coupled to flame ionisation detection, respectively. Spatio-temporal differences in maximum load, elasticity, viscosity, NSCs and fatty acid composition were found in avocados from different origins. Results of measured texture and target analytes were used to differentiate avocado fruit into definable groups using partial least squares discriminant analysis.Item Open Access CASS•E: Cranfield astrobiological stratospheric sampling experiment(2010-12-31T00:00:00Z) Naicker, L.; Grama, V. V.; Juanes-Vallejo, Clara M.; Katramados, Ioannis; Rato, Carla Cristina Pereira Salgueiro Catarino; Rix, Catherine S.; Sanchez, E.; Cullen, David C.CASS•E is a life detectionexperimentthat aims to be capable of collecting microorganisms in Earth's Stratosphere. Theexperiment will be launched on astratosphericballoon in collaboration with Eurolaunch through the BEXUS (Balloon-borneExperimentsfor Universitv Students) program from Esrange Sweden in October 2010. It essentially consists of a pump which draws air from the Stratosphere through a collection filter mechanism. Due to the low number density of microbes in the Stratosphere compared to the known levels of contamination at ground level, theexperimentincorporated Planetary Protection and Contamination Control (PP&CC) protocols in its design and construction in order to confirm that any microbes detected are trulyStratosphericin origin. Space qualified cleaning and sterilisation techniques were employed throughout Assembly Integration and Testing (AIT) as well as biobarriers which were designed to open only in the stratosphere and so prevent recontamination of the instrument alter sterilisation. The material presented here covers the design and AIT of CASS•E. Copyright ©2010 by the International Astronautical Federation. All rights reseItem Open Access Experiences in Pattern Recognition for Machine Olfaction(American Institute of Physics (AIP), 2011-09-06) Bessant, Conrad M.Pattern recognition is essential for translating complex olfactory sensor responses into simple outputs that are relevant to users. Many approaches to pattern recognition have been applied in this field, including multivariate statistics (e.g. discriminant analysis), artificial neural networks (ANNs) and support vector machines (SVMs). Reviewing our experience of using these techniques with many different sensor systems reveals some useful insights. Most importantly, it is clear beyond any doubt that the quantity and selection of samples used to train and test a pattern recognition system are by far the most important factors in ensuring it performs as accurately and reliably as possible. Here we present evidence for this assertion and make suggestions for best practice based on these findings.Item Open Access FT-infrared spectroscopic studies of lymphoma, lymphoid and myeloid leukaemia cell lines(2007-12-31T00:00:00Z) Babrah, Jaspreet; McCarthy, Keith P.; Lush, Richard; Rye, Adam D.; Bessant, Conrad M.; Stone, Nicholas; Dietrich, Schweitzer; Maryann, FitzmauriceThis paper presents a novel method to characterise spectral differences that distinguish leukaemia and lymphoma cell lines. This is based on objective spectral measurements of major cellular biochemical constituents and multivariate spectral processing. Fourier transform infrared (FT-IR) maps of the lymphoma, lymphoid and myeloid leukaemia cell samples were obtained using a Perkin-Elmer Spotlight 300 FT-IR imaging spectrometer. Multivariate statistical techniques incorporating principal component analysis (PCA) and linear discriminant analysis (LDA) were used to construct a mathematical model. This model was validated for reproducibility. Multivariate statistical analysis of FTIR spectra collected for each cell sample permit a combination of unsupervised and supervised methods of distinguishing cell line types. This resulted in the clustering of cell line populations, indicating distinct bio-molecular differences. Major spectral differences were observed in the 4000 to 800 cm- 1 spectral region. Bands in the averaged spectra for the cell line were assigned to the major biochemical constituents including; proteins, fatty acids, carbohydrates and nucleic acids. The combination of FT-IR spectroscopy and multivariate statistical analysis provides an important insight into the fundamental spectral differences between the cell lines, which differ according to the cellular biochemical composition. These spectral differences can serve as potential biomarkers for the differentiation of leukaemia and lymphoma cells. Consequently these differences could be used as the basis for developing a spectral method for the detection and identification of haematological malignancies.Item Open Access In vitro analysis of nanoparticles released from polyamide nanomaterial composites during drilling process(2011-05-10T00:00:00Z) Irfan, Adeel; Sachse, Sophia; Njuguna, James A. K.; Zhu, Huijun; Crump, DerrickNanomaterials provide a new avenue of progress into technological development. By manipulating materials on the very basic atomic and molecular levels the property of a given material can be specifically altered to suit the purpose of intended applications. However, nanomaterials (nanoparticles and nanolayers) have a more complex nature in physiochemical properties and surface reactivity than their larger counterparts. Therefore, the release of these nanomaterials as dust during crushing or drilling may lead to serious health hazards for humans and the surrounding environment. This study, supported by the NEPHH (Nanomaterial-related Environmental Pollution Health Hazards), addresses two important questions about nanomaterials: - Whether nanomaterials can be released from physical process of nanoproducts. - Toxicity potential of nanodusts generated from nanoproducts in comparison with reference products. Both questions address a massive gap in knowledge for toxicity and more specifically nanomaterial toxicity.Item Open Access Spatio-temporal postharvest changes in texture and fatty acid profiles in avocado fruit from different origins(2008-06-29T00:00:00Z) Landahl, Sandra; Terry, Leon A; Meyer, Marjolaine D.The degree of ripeness of many climacteric fruits, such as avocado, can be correlated with fleshfirmness and other rheological properties. However, there remains a paucity of information on not only thepostharvest changes in texture of avocado fruit from different origins, but also on the spatial variation in texturewithin individual fruit. In addition, the relationship between changes in texture and lipid profile of fruit tissueduring postharvest ripening is unknown.The aim of the present study was to assess and discriminate between avocado cv. Hass fruit from threedifferent origins (viz. Spain, Peru and Chile) on the basis of temporal and spatial changes in both texture andfatty acid profiles of fruit flesh. Texture of different horizontally-cut slices from individual fruit within aconsignment was measured during ripening using a previously unreported technique. Maximum load, elasticityand viscosity of fruit tissue was measured using an Instron 5542 universal testing machine fitted with either a500 N or 5 N load cell. The same fruit slice was immediately snap-frozen in liquid nitrogen and freeze-driedprior to subsequent extraction, identification and determination of fatty acid methyl esters (FAME) profiles usinggas chromatography coupled to flame ionization detection (GC-FID). The results were used to differentiate avocado fruit into definable groups using partial least squares discriminant analysis. Significant differences inmaximum load, elasticity, viscosity were found in avocado fruit flesh during ripening, and between origins andto a lesser extent between different locations within the fruit. Lipid profiles showed dissimilar compositionaccording to origin and changed slightly from apex to base. The textural changes and lipid profile in avocadofruit is therefore related to origin and the spatial variation within individual fruit.Item Open Access Textural, biochemical and micro-structural changes in mesocarp tissue of imported avocado from Peru during ripening(2010-12-31T00:00:00Z) Landahl, Sandra; Terry, Leon A.Avocado (Persea americana Mill.) fruit is a valuable product and notorious for the difficulties encountered in determining quality. Typically the degree of ripeness of many climacteric fruits, such as avocado, is measured by assessing flesh firmness. The aim of the presented work was to elucidate the temporal and spatial changes in texture, biochemistry and micro-structure in different avocado tissues from the same fruit. Fruit were first treated with ethylene and then ripened at 12°C. Samples were taken four times over 10 days storage. Maximum load and viscoelasticity of horizontally-cut slices from fruit (n=24) imported from Peru were measured during ripening. These texture parameters were measured using an Instron 5542 universal testing machine fitted with a 500 N or 5 N load cell. Non-structural carbohydrates (NSCs; viz. sucrose, mannoheptulose, perseitol) and fatty acid methyl esters from the same samples were identified and quantified using standard HPLC coupled to evaporative light scattering detection and gas chromatography coupled to flame ionisation detection, respectively. Samples taken from adjacent mesocarp tissue slices were examined: each specimen included the sclerenchymatic exocarp, fleshy mesocarp and endocarp including the coat of the seed. An analysis of variance was performed to elucidate the change of parameters during fruit ripening. NSC content and textural properties changed during ripening and showed spatial heterogeneity within individual fruit. Micro-structural changes were evident during the latter stages of ripening and coincided with fruit softening and degradation in mannoheptulose. These findings might be used to enhance quality monitoring of imported avocado fruit.