Staff publications - Cranfield Health
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Browsing Staff publications - Cranfield Health by Subject "Analysis Type A trichothecenes Diode array Cereals performance liquid-chromatography diode-array detection gas-chromatography mass-spectrometry immunoaffinity cleanup fluorescence detection fusarium-langsethiae retention indexes b-trichothecene cereals"
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Item Open Access Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media(Elsevier Science B.V., Amsterdam., 2010-08-01T00:00:00Z) Medina-Vayá, Ángel; Valle-Algarra, Francisco M.; Jimenez, Misericordia; Magan, NareshA LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol: water (80:20, v/v) for 90 min, drying with N-2 and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier. (C) 2010 Elsevier B.V. All rights reserved.