Staff publications - Cranfield Health

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Browsing Staff publications - Cranfield Health by Subject "Amperometric enzyme electrode"

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    Flexible Ultrathin PolyDVB/EVB Composite Membranes for the Optimization of a Lactate Sensor.
    (John Wiley & Sons, Ltd, 2007-01-01T00:00:00Z) Bridge, Kerry; Davis, Frank; Collyer, Stuart D.; Higson, Seamus P. J.
    An ultrathin composite membrane has been developed as the outer covering barrier in a model amperometric lactate oxidase enzyme electrode. The membrane was formed by cathodic electropolymerization of divinylbenzene/ethylvinylbenzene at the surface of a gold coated polyester support membrane. Permeability coefficients were determined for O2 and lactate across membranes with a range of polymer thicknesses. Anionic interferents (such as ascorbate) were screened from the working electrode by the composite membrane. The composite enzyme electrode showed an increased working concentration range and extended linearity of responses in comparison to an uncoated enzyme electrode.
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    ItemOpen Access
    Flexible Ultrathin PolyDVB/EVB Composite Membranes for the Optimization of a Whole Blood Glucose Sensor.
    (John Wiley & Sons, Ltd, 2007-01-01T00:00:00Z) Bridge, Kerry; Davis, Frank; Collyer, Stuart D.; Higson, Seamus P. J.
    An ultrathin composite membrane has been developed as the outer covering barrier in a model amperometric glucose oxidase enzyme electrode. The membrane was formed by cathodic electropolymerization of divinylbenzene/ethylvinylbenzene at the surface of a gold coated polyester support membrane. Permeability coefficients were determined for O2 and glucose across membranes with a range of polymer thicknesses. Anionic interferents (such as ascorbate), were screened from the working electrode via a charge exclusion mechanism. The enzyme electrode showed an initial 10% signal drift when first exposed to whole human blood over a period of 2 hours, after which responses remained essentially stable. Whole blood patient glucose determinations yielded a correlation coefficient of r2=0.99 compared to standard hospital analyses.