Staff publications - Cranfield University at Silsoe

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Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.
(Elsevier Science B.V., Amsterdam., 2009-01-01T00:00:00Z) Barton, Andrew C.; Collyer, Stuart D.; Davis, Frank; Garifallou, Goulielmos-Zois; Tsekenis, Georgios; Tully, Elizabeth; O'Kennedy, Richard; Gibson, Tim; Millner, Paul A.; Higson, Seamus P. J.
This paper describes the development and characterisation of labeless immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin (BSA). Commercial screen-printed carbon electrodes were used as the basis for the sensors. Two methods were used to immobilise antibodies at the electrode surface. Aniline was electropolymerised onto these electrodes to form a thin planar film of conductive polyaniline; the polyaniline film was then utilised as a substrate to immobilise biotinylated anti-digoxin using a classical avidin- biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene) was electrodeposited onto the carbon electrodes and this modified surface was then sonochemically ablated to form an array of micropores. A second electropolymerisation step was then used to co-deposit conductive polyaniline along with antibodies for BSA within these pores to produce a microarray of polyaniline protrusions with diameters of several μm, containing entrapped anti- BSA