Browsing by Author "Zhang, Juankun"

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    A label free electrochemical nanobiosensor study
    (Taylor & Francis, 2009-01) Zhang, Juankun; Dong, Shanmu; Lu, Jinhui; Turner, Anthony P. F.; Fan, Qingjie; Jia, Shiru; Yang, Hongjiang; Qiao, Changsheng; Zhou, Hao; He, Guowei
    Nano-porous silicon (PS) is an attractive material for incorporation into biosensors, because it has a large surface area combined with the ability to generate both optical and electrical signals. In this paper, we describe a label-free nanobiosensor for bovine serum albumin (BSA). Nano-porous silicon produced in our laboratory was functionalized prior to immobilization of anti-BSA antibody on the surface. Reaction with BSA in phosphate buffered saline (PBS) buffer resulted in an impedance change which was inversely proportional to the concentration of the analyte. The system PBS buffer/antigen-antibody/PS constitutes an electrolyte-insulator-semiconductor (EIS) structure, thus furnishing an impedance EIS nanobiosensor. The linear range of the sensor was 0-0.27 mg mL-1 and the sensitivity was less than 10 µg mL-1.
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    Nano-Porous Light-Emitting Silicon Chip as a Potential Biosensor Platform
    (Taylor and Francis, 2008-01) Zhang, Juankun; Zhang, Weiliang; Dong, Shanmu; Turner, Anthony P. F.; Fan, Qingjie; Jia, Shiru
    Nano-porous silicon (PS) offers a potential platform for biosensors with benefits both in terms of light emission and the large functional surface area. A light emitting PS chip with a stable and functional surface was fabricated in our laboratory. When protein was deposited on it, the light emission was reduced in proportion to the protein concentration. Based on this property, we developed a rudimentary demonstration of a label-free sensor to detect bovine serum albumin (BSA). A serial concentration of BSA was applied to the light chip and the reduction in light emission was measured. The reduction of the light intensity was linearly related to the concentration of the BSA at concentrations below 10-5 M. The detection limit was 8×10-9 M.