Browsing by Author "Tyrrell, Sean R."

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    Can chemical and molecular biomarkers help discriminating industrial, rural and urban environments?
    (Cranfield University, 2018-02-05 10:48) Coulon, Frederic; Tyrrell, Sean R.; Garcia Alcega, Sonia
    Underlying data to study the feasibility of differentiate urban, rural and industrial areas between seasons with chemical and molecular markers such as MVOCs and PLFAs
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    Conceptual energy and water recovery system for self-sustained nano membrane toilet
    (Elsevier, 2016-08-12) Hanak, Dawid P.; Kolios, Athanasios; Onabanjo, Tosin; Wagland, Stuart Thomas; Patchigolla, Kumar; Fidalgo Fernandez, Beatriz; Manovic, Vasilije; McAdam, Ewan J.; Parker, Alison; Williams, Leon; Tyrrell, Sean R.; Cartmell, Elise
    With about 2.4 billion people worldwide without access to improved sanitation facilities, there is a strong incentive for development of novel sanitation systems to improve the quality of life and reduce mortality. The Nano Membrane Toilet is expected to provide a unique household-scale system that would produce electricity and recover water from human excrement and urine. This study was undertaken to evaluate the performance of the conceptual energy and water recovery system for the Nano Membrane Toilet designed for a household of ten people and to assess its self-sustainability. A process model of the entire system, including the thermochemical conversion island, a Stirling engine and a water recovery system was developed in Aspen Plus®. The energy and water recovery system for the Nano Membrane Toilet was characterised with the specific net power output of 23.1 Wh/kgsettledsolids and water recovery rate of 13.4 dm3/day in the nominal operating mode. Additionally, if no supernatant was processed, the specific net power output was increased to 69.2 Wh/kgsettledsolids. Such household-scale system would deliver the net power output (1.9–5.8 W). This was found to be enough to charge mobile phones or power clock radios, or provide light for the household using low-voltage LED bulbs.
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    Effect of temperature on bacteriophage-mediated lysis efficiency with a special emphasis on bacterial temperature history
    (University of Mohammed Premier, Oujda, Morocco, 2022-09-30) Ameh, Ekwu Mark; Nocker, Andreas; Tyrrell, Sean R.; Harris, Jim A.; Orlova, Elena V.; Ignatiou, Athanasios
    Despite the great potential of phages as biocontrol agents, there is much uncertainty about the environmental factors influencing lysis efficiency. In this study we investigated the effect of temperature using three distinct lytic E. coli phages that were isolated from a single environmental water sample. All three were identified as dsDNA phages belonging to the Myoviridae family. Whereas the optimal growth temperature of E. coli is well known to be 37 ˚C and exposure of phages (prior to mixing with bacteria) to temperatures between 4 and 37˚C did not affect their infectivity, plaque sizes and numbers greatly decreased with increasing incubation temperature (20˚C, 30˚C, 37˚C) of the phage-host mix. At 37˚C, no visible plaques were observed. Results suggest that temperature sensitivity of the phage-host interaction is distinct from the temperature susceptibility of the two players and corroborate previous reports that highest lysis rates are obtained at temperatures approximate with ambient conditions of the phage environment. Infectivity was however found not only to depend on the incubation temperature of the phage-host mix, but also on the bacterial temperature history. Moreover, exposure of bacteria to heat stress prior to phage challenge resulted in a phage-resistant phenotype raising the question whether bacterial pathogens shed from warm-blooded hosts might be less susceptible to phages adapted to environmental temperature conditions.
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    Microtopographic enhancement of land-based wastewater treatment
    (Cranfield University, 2016-09) Tyrrell, Sean R.; Hess, Tim M.; Tyrrel, Sean
    There is a regulatory tension within wastewater treatment, between the requirement to meet tightening consents and the need to reduce the carbon footprint of treatment processes. With 75% of wastewater treatment works serving populations of less than 2,000, low-energy tertiary treatment options suitable to small rural works need to be developed. One option that lends itself particularly well to small works is land-based wastewater treatment (LBWWT). The aim of this research was to evaluate the role of LBWWT in the UK water industry and investigate the impact ridge-and-furrow enhanced microtopography (MT) may have upon a particular type of LBWWT - slow-rate (SR) infiltration. This was achieved through meeting three objectives. Firstly, the use of LBWWT was reviewed and assessed. Secondly, the impact of ridge-and-furrow enhanced MT upon the vegetation diversity and nutrient removal of a SR- LBWWT was established by means of a three year field trial. Finally, the cost- effectiveness of SR-LBWWT and the impact of ridging and furrow irrigation upon cost-effectiveness were evaluated using Cost-Effectiveness Analysis (CEA). The first objective comprised of a review of the historical and current use of LBWWT, a review of the relevant changing legislation to identify what may be required of LBWWT and an assessment of LBWWT’s potential to meet these requirements. The result of the evaluation found that, based upon the literature, SR-LBWWT is ‘fit-for-purpose’ as tertiary treatment for small treatment works. To meet the second objective, a SR-LBWWT system trial was established at a small wastewater treatment works in Knowle, Hampshire. The trial consisted of three clay-loam grass plots irrigated with secondary treated effluent. There were two configurations of trial plot - flat and ridge-and-furrowed. Effluent (sub- surface soil water) nutrient concentrations were monitored as was vegetation diversity. In addition a number of physical, hydrological and biogeochemical parameters were monitored and hydrological modelling carried out. Mean nutrient removal performances of 90% for ammonia, 72% for nitrate, and 91% for phosphate were observed with the ridge-and-furrowed plot. Ridging and furrow irrigation was found to not have a significantly detrimental effect upon the trial plots’ removal performance for ammonia, nitrate or phosphate. Extrapolation modelling suggested, however, that this would not be the case for LBWWT systems on predominantly clay or sand soils. Ridging and furrow irrigation was found to have a statistically significant positive effect upon the vegetation diversity of the LBWWT trial plots; with mean final year Shannon-Wiener values of 0.96 and 0.69, for the ridge-and-furrowed and non-ridged plots, respectively. For the final objective, analysis found that SR-LBWWT are cost-effective when compared to horizontal sub-surface flow constructed wetlands (HSSFCW), an established low-energy treatment option. Mean cost-effectiveness ratio values of £208.5 and £262.7 per % effectiveness were observed for LBWWT and HSSFCW, respectively. Following the field trial CEA was extended to include ridge-and-furrowed SR-LBWWT systems. This found that ridging and furrow irrigation improves the cost-effectiveness of SR-LBWWT serving small populations, reducing the mid cost-effectiveness ratio to £193 per % effectiveness. This is a result of the cost-reducing effect of ridge-and-furrowing over laser-level grading; and based upon the findings of the trial that ridging and furrow irrigation can be achieved (in clay-loam soil slow-rate systems) without significant detriment to the water treatment effectiveness of LBWWT. The main conclusions of this thesis are: that SR-LBWWT has a role to play in the UK water industry, as tertiary treatment for small wastewater treatment works. That SR-LBWWT is cost-effective in relation to HSSFCW. That ridging and furrow irrigation increases that cost-effectiveness by reducing the construction and operational costs. That ridging and furrow irrigation can be employed without significant detriment to a SR-LBWWT system’s water treatment performance. And finally, that ridging and furrow irrigation can have a positive impact upon the establishment vegetation diversity of a SR-LBWWT system.