Browsing by Author "Sarkar, P."

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    Electrochemical Immunoassay for Free Prostate Specific Antigen (f-PSA) Using Magnetic Beads
    (Wiley InterScience, 2008) Sarkar, P.; Ghosh, D.; Bhattacharyay, D.; Setford, S. J.; Turner, Anthony P. F.
    Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of f-PSA using magnetic beads on a three-electrode screen-printed sensor. A magnetic bead enzyme linked immunosorbent assay (ELISA) was performed in a cuvette. Following the immunoassay, magnetic beads were recovered by a magnetic concentrator and transferred on the working electrode of the 3-electrode assembly. The amperometric response, a measure of the amount of residual enzyme activity on the beads and hence the concentration of analyte in solution, was determined by addition of enzyme substrate. The device has a detection limit of <0.1 ng mL-1 f-PSA and a linear range of 0 to 1 ng mL-1 f-PSA.