Browsing by Author "Riba, Amar"

Now showing 1 - 2 of 2
  • Thumbnail Image
    ItemOpen Access
    Incidence of mycobiota and aflatoxin B1 in Algerian feed
    (Inderscience, 2022-03-29) Bouti, Karima; Mimoune, Nouara Ait; Mokrane, Salim; Djemouai, Nadjette; Verheecke-Vaessen, Carol; Mathieu, Florence; Riba, Amar
    The presence of fungi and aflatoxin B1 (AFB1) in 101 animal feed samples randomly collected from different vendors and factories in Algeria was investigated. For fungi, the main genera isolated were Aspergillus, Penicillium and Fusarium. Furthermore, the 459 strains of Aspergillus section Flavi were screened for their ability to produce aflatoxins and cyclopiazonic acid. 49% of the strains produced AFB1. The highest incidence of aflatoxigenic strains was recorded in maize (61%) and ground poultry feed (60%). The presence of AFB1 in feed samples was evaluated using HPLC-FLD. The obtained data showed that 36.6% of samples were contaminated in the range of 0.34 to 171.06 μg/kg. Six samples exceeded the Algerian maximum limit of 20 µg/kg for AFB1. This study highlights the potential presence of aflatoxigenic strains belonging to section Flavi and AFB1 in animal feed at post-harvest in Algeria, strategic information for the Algerian policies makers.
  • Thumbnail Image
    ItemOpen Access
    Polyphasic characterization of Aspergillus section Flavi isolated from animal feeds in Algeria
    (Wiley, 2019-12-13) Bouti, Karima; Verheecke-Vaessen, Carol; Mokrane, Salim; Meklat, Atika; Djemouai, Nadjette; Sabaou, Nasserdine; Mathieu, Florence; Riba, Amar
    In Algeria, little information is available on the population structure of Aspergillus section Flavi in raw materials and resultant animal feeds. A total of 172 isolates belonging to Aspergillus section Flavi were recovered from 57 animal feeds and identified on the basis of macro and micro‐morphological characters, mycotoxin production and genetic relatedness. For the molecular analysis, sequencing of the calmodulin gene (CaM) and the internal transcribed spacer (ITS) regions were performed for representative isolates. Four distinct morphotypes were distinguished: Aspergillus flavus (78.5%), Aspergillus tamarii (19.2%), Aspergillus parasiticus (1.7%), and Aspergillus alliaceus (0.6%). All A. flavus isolates were of the L type and no correlation between sclerotia production and aflatoxigenicity was observed. Our results showed that 68% of the A. flavus strains produced aflatoxins B (AFB), and 72.7% were cyclopiazonic acid (CPA) producers. The three isolates of A. parasiticus were able to produce AFB and aflatoxins G but not CPA whereas, all the strains of A. tamarii produced only CPA. The obtained results revealed the presence of different species of Aspergillus section Flavi, among which were aflatoxin producers. This study provides evidence useful for considerations in aflatoxin control strategies.