Browsing by Author "Pundir, Anushka"
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Item Open Access Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity(Elsevier, 2020-08-22) Baral, Pratibha; Pundir, Anushka; Kumar, Vinod; Kurmi, Akhilesh K.; Agrawal, DeeptiSugarcane bagasse (SCB) is anticipated to emerge as a potential threat to waste management in India on account of cheap surplus energy options and lower incentives through its co-generation. Through biotechnological intervention, the efficient utilization of SCB is seen as an opportunity. The present study aimed towards expeditious production of concentrated glucose-rich hydrolysate from SCB. Alkali pretreated biomass was chosen for hydrolysis with a new generation cellulase cocktail, Cellic CTec2 dosed at 25 mg g−1 glucan content. A two-step (9% + 9%) substrate feeding strategy was adopted with a gap of an hour, and saccharification was terminated in three different ways. Irrespective of the methods employed for termination, ∼84.5% cellulose was hydrolyzed releasing ≥100 g L−1 glucose from 18% biomass. Direct use of glucose-rich filtrates yielded 69.2 ± 2.5 g L−1 of L (+) lactic acid (LA) using thermophilic Bacillus coagulans NCIM 5648. The best-attained glucose and LA productivities during separate hydrolysis and fermentation (SHF) in the present study were 5.27 and 2.88 g L−1 h−1, respectively. A green and sustainable process is demonstrated for the production of industrially relevant sugars from SCB at high productivity and its valorization to bio-based LA.Item Open Access Salting-out assisted solvent extraction of L (+) lactic acid obtained after fermentation of sugarcane bagasse hydrolysate(Elsevier, 2021-04-18) Baral, Pratibha; Pundir, Anushka; Kurmi, Akhilesh; Singh, Raghuvir; Kumar, Vinod; Agrawal, DeeptiLactic acid is among the twelve platform chemicals produced from inexpensive and renewable feedstocks such as lignocellulosic biomass. The present study illustrates salting-out assisted solvent extraction of L (+) lactic acid, derived from sugarcane bagasse hydrolysate. Screening studies with various extractants and diluents revealed that a combination of tri-n-butyl phosphate and ethyl acetate yielded the best results and extracted 59.63 ± 1.28% lactic acid from the dilute fermentation broth adjusted to a pH of 1.6 ± 0.2. Various inorganic salts were screened to enhance extraction efficiency further. The addition of 60% (w/v) ammonium sulfate improved the lactic acid extraction by 36.17%. This salt concentration successfully extracted 85.95 ± 0.44% lactic acid to the organic phase under optimized conditions from the fermentation broth at a pH of ~ 2.5 containing 40–100 g L−1 lactic acid. Recovery of > 80% salt is also shown using chilled acetone, which upon reuse showed a nominal decline of 3.3% during extraction. Thus an eco-friendly approach using a green solvent like ethyl acetate with mild operating conditions is demonstrated in the present study.