Browsing by Author "Nagarajan, Sanjay"
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Item Open Access Integrated biorefineries for repurposing of food wastes into value-added products(Elsevier, 2022-09-01) Narisetty, Vivek; Adlakha, Nidhi; Singh, Navodit Kumar; Dalei, Sudipt Kumar; Prabhu, Ashish A.; Nagarajan, Sanjay; Kumar, A. Naresh; Nagoth, Joseph Amruthraj; Kumar, Gopalakrishnan; Singh, Vijai; Kumar, VinodFood waste (FW) generated through various scenarios from farm to fork causes serious environmental problems when either incinerated or disposed inappropriately. The presence of significant amounts of carbohydrates, proteins, and lipids enable FW to serve as sustainable and renewable feedstock for the biorefineries. Implementation of multiple substrates and product biorefinery as a platform could pursue an immense potential of reducing costs for bio-based process and improving its commercial viability. The review focuses on conversion of surplus FW into range of value-added products including biosurfactants, biopolymers, diols, and bioenergy. The review includes in-depth description of various types of FW, their chemical and nutrient compositions, current valorization techniques and regulations. Further, it describes limitations of FW as feedstock for biorefineries. In the end, review discuss future scope to provide a clear path for sustainable and net-zero carbon biorefineries.Item Open Access Process optimization for recycling of bread waste into bioethanol and biomethane: a circular economy approach(Elsevier, 2022-05-28) Narisetty, Vivek; Nagarajan, Sanjay; Gadkari, Siddharth; Ranade, Vivek V.; Zhang, Jingxin; Patchigolla, Kumar; Bhatnagar, Amit; Awasthi, Mukesh Kumar; Pandey, Ashok; Kumar, VinodBread is the second most wasted food in the UK with annual wastage of 292,000 tons. In the present work, bread waste (BW) was utilized for fermentative production of ethanol by Saccharomyces cerevisiae KL17. Acidic and enzymatic saccharification of BW was carried out resulting in the highest glucose release of 75 and 97.9 g/L which is 73.5 and 95.9% of theoretical yield, respectively. The obtained sugars were fermented into ethanol initially in shake flask followed by scale up in bioreactor in batch and fed-batch mode. In the fed-batch mode of cultivation, the maximum ethanol titers of 111.3, 106.9, and 114.9 g/L with conversion yield and productivity of 0.48, 0.47, and 0.49 g/g, and 3.1, 3.0, and 3.2 g/L.h was achieved from pure glucose, glucose-rich acidic and enzymatic hydrolysates, respectively. Further to improve the process economics, the solid residues after acidic (ABW) and enzymatic (EBW) hydrolysis of BW along with respective fermentation residues (FR) obtained after the ethanol production were pooled and subjected to anaerobic digestion. The solid residue from ABW + FR, and EBW + FR yielded a biochemical methanation potential (BMP) of 345 and 379 mL CH4/g VS, respectively. Life cycle assessment of the process showed that the total emissions for ethanol production from BW were comparable to the emissions from more established feedstocks such as sugarcane and maize grain and much lower when compared to wheat and sweet potato. The current work demonstrates BW as promising feedstock for sustainable biofuel production with the aid of circular biorefining strategy. To the authors knowledge, this is the first time, such a sequential system has been investigated with BW for ethanol and biomethane production. Further work will be aimed at ethanol production at pilot scale and BMP will be accessed in a commercial anaerobic digester.Item Open Access Sugarcane bagasse based biorefineries in India: potential and challenges(Royal Society of Chemistry, 2020-11-10) Konde, Kakasaheb S.; Nagarajan, Sanjay; Kumar, Vinod; Patil, Sanjay V.; Ranade, Vivek V.Sugarcane bagasse (SCB) is one of the world's most abundant agricultural residues and in an Indian context, ∼100 million tonnes per annum is produced. The current use of SCB is restricted to the cogeneration of steam and power; however considering its potential, cogeneration is not the best valorisation route. Furthermore, with falling electricity prices and reducing global sugar prices due to excess sugar stock, it is inevitable that the waste generated (SCB) by sugar mills are utilised for generating revenue sustainably. With this background, this review aims to put forth a biorefinery perspective based on SCB feedstock. Biogas and bioethanol are the Government of India's current focus with policies and subsidies clearly pointing towards a sizeable future market. Therefore, alongside these biofuels, high-value chemicals such as xylitol, succinic acid and lactic acid were identified as other desired products for biorefineries. This review firstly discusses SCB pre-treatment options based on end applications (saccharification or anaerobic digestion, AD). Next, state-of-the-art for each of these aspects was reviewed and our perspective on a profitable biorefinery is presented. We propose an AD based biorefinery where vortex-based hydrodynamic cavitation was found to be the best choice for pre-treatment. AD is considered not only a bioprocess for energy production here but also a ‘pre-treatment’, where partial conversion of holocellulose leads to a digestate rich in a loosened fibre matrix. This digestate rich in cellulose can be enzymatically hydrolysed and further valorised biochemically. This approach would be cost effective and provide a sustainable waste management route for sugar mills.Item Open Access Supporting data for the publication' High-Level fermentative production of Lactic acid from bread waste under Non-sterile conditions with a circular biorefining approach and zero waste discharge(Cranfield University, 2022-01-13 18:05) Cox, Rylan; Kumar, Vinod; Narisetty, Vivek; Salonitis, Konstantinos; Agarwal, Deepti; V. Ranade, Vivek; Nagarajan, Sanjay; Venus, JoachimBread waste (BW) is a severe solid waste management problem in Europe. The current study demonstrates an environment-friendly solution by valorising BW into lactic acid (LA) and the corresponding solid residues generated during hydrolysis and fermentation to biogas. To this end, BW was saccharified through acidic and enzymatic hydrolysis, and the hydrolysate obtained was used for LA fermentation under non-sterile conditions using thermophilic Bacillus coagulans DSM1. Maximum glucose concentration achieved during acid hydrolysis with 2% (v/v) acid loading and 20% (w/v) solid loading was 67.9 g/L glucose, with a yield of 0.34 g/g BW. The LA accumulated with concentrated BW acid hydrolysate was 102.4 g/L with yield and productivity of 0.75 g/g and 1.42 g/L. h, respectively. For enzymatic hydrolysis, three commercial amylase preparations (Amyloglucosidase, Spirizyme, Dextrozyme) were employed. The highest glucose release (98.6 g/L) and yield (0.49 g glucose/g BW) was attained with Dextrozyme from Novozymes. The fed-batch fermentation by B. coagulans was conducted, using commercial glucose and glucose-rich BW hydrolysate from Dextrozyme. The LA titer, yield and productivity obtained with pure glucose were 222.7 g/L, 0.92 g/g and 1.86 g/L.h, respectively, whereas BW hydrolysate (BWH) resulted in 155.4 g/L LA, with a conversion yield and productivity of 0.85 g/g glucose and 1.30 g/L. h, respectively. Further to the LA biosynthesis, the solid residues generated during hydrolysis and fermentation were subjected to biogas generation, resulting in 553 mL CH4/g volatile solids under batch mode. This massive LA titer amassed under non-sterile conditions and integrated biogas production using fermented residues demonstrates a high potential for an integrated biorefinery based on BW.