Browsing by Author "Mokrane, Salim"
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Item Open Access Biological activities of Streptomyces sp. Bts40 isolated from the rhizosphere of Artemisia herba-alba Asso(Universitatea din Oradea, 2022-01-04) Djemouai, Nadjette; Meklat, Atika; Gaceb-Terrak, Rabéa; Oulad Hadj Youcef, Khadidja; Nacer, Asma; Mokrane, Salim; Bouras, Noureddine; Verheecke-Vaessen, CarolActinobacteria isolated from the rhizosphere of plants are of interest as they produce a diverse range of molecules, such as antibiotics and enzymes. This study investigates the antibacterial activity, plant growth-promoting (PGP) abilities as well as the production of extracellular enzymes by the actinobacterial strain BTS40. This strain was isolated from the rhizospheric soil of the medicinal plant Artemisia herba-alba Asso that was naturally grown in a semi-arid environment. Morphological characteristics showed that the strain BTS40 belongs to the genus Streptomyces. Analysis of BTS40’s 16S rRNA gene sequence showed 99.45% similarity to Streptomyces alboniger NRRL B-1832T, in the EzTaxon database. This actinobacterium showed only antibacterial activity against Gram-positive bacteria. The strain also showed potential multiple traits for plant growth promotion and hydrolysis of enzymes. Hence, this study reveals that strain BTS40 has multiple PGP traits and produces many extracellular hydrolytic enzymes.Item Open Access Incidence of mycobiota and aflatoxin B1 in Algerian feed(Inderscience, 2022-03-29) Bouti, Karima; Mimoune, Nouara Ait; Mokrane, Salim; Djemouai, Nadjette; Verheecke-Vaessen, Carol; Mathieu, Florence; Riba, AmarThe presence of fungi and aflatoxin B1 (AFB1) in 101 animal feed samples randomly collected from different vendors and factories in Algeria was investigated. For fungi, the main genera isolated were Aspergillus, Penicillium and Fusarium. Furthermore, the 459 strains of Aspergillus section Flavi were screened for their ability to produce aflatoxins and cyclopiazonic acid. 49% of the strains produced AFB1. The highest incidence of aflatoxigenic strains was recorded in maize (61%) and ground poultry feed (60%). The presence of AFB1 in feed samples was evaluated using HPLC-FLD. The obtained data showed that 36.6% of samples were contaminated in the range of 0.34 to 171.06 μg/kg. Six samples exceeded the Algerian maximum limit of 20 µg/kg for AFB1. This study highlights the potential presence of aflatoxigenic strains belonging to section Flavi and AFB1 in animal feed at post-harvest in Algeria, strategic information for the Algerian policies makers.Item Open Access Polyphasic characterization of Aspergillus section Flavi isolated from animal feeds in Algeria(Wiley, 2019-12-13) Bouti, Karima; Verheecke-Vaessen, Carol; Mokrane, Salim; Meklat, Atika; Djemouai, Nadjette; Sabaou, Nasserdine; Mathieu, Florence; Riba, AmarIn Algeria, little information is available on the population structure of Aspergillus section Flavi in raw materials and resultant animal feeds. A total of 172 isolates belonging to Aspergillus section Flavi were recovered from 57 animal feeds and identified on the basis of macro and micro‐morphological characters, mycotoxin production and genetic relatedness. For the molecular analysis, sequencing of the calmodulin gene (CaM) and the internal transcribed spacer (ITS) regions were performed for representative isolates. Four distinct morphotypes were distinguished: Aspergillus flavus (78.5%), Aspergillus tamarii (19.2%), Aspergillus parasiticus (1.7%), and Aspergillus alliaceus (0.6%). All A. flavus isolates were of the L type and no correlation between sclerotia production and aflatoxigenicity was observed. Our results showed that 68% of the A. flavus strains produced aflatoxins B (AFB), and 72.7% were cyclopiazonic acid (CPA) producers. The three isolates of A. parasiticus were able to produce AFB and aflatoxins G but not CPA whereas, all the strains of A. tamarii produced only CPA. The obtained results revealed the presence of different species of Aspergillus section Flavi, among which were aflatoxin producers. This study provides evidence useful for considerations in aflatoxin control strategies.