Browsing by Author "Jin, Mingjie"
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Item Open Access Carbon emissions and decarbonisation: the role and relevance of fermentation industry in chemical sector(Elsevier, 2023-10-07) Agrawal, Deepti; Awani, Kelvin; Nabavi, Seyed Ali; Balan, Venkatesh; Jin, Mingjie; Aminabhavi, Tejraj M.; Dubey, Kashyap Kumar; Kumar, VinodFermentation industry is emerging as sustainable technological alternative to cater the production of various chemical building blocks which are commercially manufactured by petrochemical route. The primary reason for this major transition is global commitment towards decarbonisation of chemical sector, as their conventional fossil-based routes pose serious environmental threat. For instance, in 2022, the direct carbon dioxide (CO2) emission during synthesis of primary chemicals accounted for ∼ 920 Mt. CO2 is one of the prominent greenhouse gases (GHG’s), contributing majorly towards global warming effect and drastic climate change. Fermentation industry largely thrives on exploiting fermentable and organic carbon derived from edible and/or non-edible biomass and transforming them to valorised products using microbial cell factories. Therefore, the production of bio-based chemicals via this route is often associated with low or zero-carbon footprint, resulting in either carbon neutral or carbon negative products. This review focuses on different types of fermentative processes and their impact on carbon release and decarbonisation. It further discusses the relevance and contribution of fermentation industry as well as biological processes to provide a sustainable solution towards decarbonisation of chemical sector. Further, it showcases the advantages of some commercial proven and/or pipeline bio-based products over their conventional competitor fossil-based products, especially from an environmental viewpoint. Finally, advantages of biogenic CO2 from fermentation industry over other sources and CO2 removal from fermentation as a platform for carbon offsetting are covered.Item Embargo Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis(Elsevier, 2024-03-18) Lu, Minrui; Sha, Yuanyuan; Kumar, Vinod; Xu, Zhaoxian; Zhai, Rui; Jin, MingjieAs a sustainable and renewable alternative to petroleum fuels, advanced biofuels shoulder the responsibility of energy saving, emission reduction and environmental protection. Traditional engineering of cell factories for production of advanced biofuels lacks efficient high-throughput screening tools and regulating systems, impeding the improvement of cellular productivity and yield. Transcription factor-based biosensors have been widely applied to monitor and regulate microbial cell factory products due to the advantages of fast detection and in-situ screening. This review updates the design and application of transcription factor-based biosensors tailored for advanced biofuels and related intermediates. The construction and genetic parts selection principle of biosensors are discussed. Strategies to enhance the performance of biosensor, including regulating promoter strength and RBS strength, optimizing plasmid copy number, implementing genetic amplifier, and modulating the structure of transcription factor, have also been summarized. We further review the application of biosensors in high-throughput screening of new metabolic engineering targets, evolution engineering, confirmation of protein function, and dynamic regulation of metabolic flux for higher production of advanced biofuels. At last, we discuss the current limitations and future trends of transcription factor-based biosensors.