Browsing by Author "Han, Wenchao"

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    Association of adverse fetal outcomes with placental inflammation after oral gestational exposure to hexafluoropropylene oxide dimer acid (GenX) in Sprague-Dawley rats
    (Elsevier, 2023-09-16) Lv, Di; Liu, Hongyun; An, Qi; Lei, Chengwei; Wang, Yanxuan; Sun, Jin; Li, Chuanhai; Lin, Yongfeng; Dong, Qing; Yang, Zhugen; Che, Kui; Liu, Wendong; Han, Wenchao
    Hexafluoropropylene oxide dimer acid (HFPO-DA), known as “GenX” for its trade name, is gradually taking the place of Perfluorooctanoic acid (PFOA). However, there is a poor understanding of the developmental effects of GenX. This study aims to explore whether GenX produces adverse effects on offspring development in Sprague-Dawley (SD) rats and the underlying mechanisms. Pregnant rats were orally administered with GenX (0, 1, 10 and 100 mg/kg/day) from gestational 0.5–19.5 days. Experimental data showed that the exposure to GenX resulted in increased rats’ gestational weight gain, whereas both body weight and body length of their fetuses born naturally were significantly reduced. This could contribute to the developmental delays of fetal body weight, body length and tail length from postnatal 1–21 days. Histopathological evaluation of placenta indicated that GenX exposure led to neutrophil infiltration in decidual zone and congestion in labyrinth zone. Moreover, placental proteomics showed changes at the expression levels of the inflammation-related proteins in the Rap1 signaling pathway. In conclusion, gestational exposure to GenX induced fetal intrauterine and extrauterine development retardation in SD rats. Placental inflammation may play a key role in this process through the Rap1 signaling pathway.
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    CRISPR-enabled sensors for rapid monitoring of environmental contaminants
    (Elsevier, 2025-03-01) Wang, Yiting; Pan, Yuwei; Han, Wenchao; Rossi, Carla Spatola; Hui, Qingxin; Guo, Ying; Owoseni, Mojisola Christiana; McAdam, Ewan; Yong, Yang-Chun; Wang, Baojun; Yang, Zhugen
    There is increasing attention on the impacts of contaminants on environmental and human health. To better understand the potential threat to ecosystems and human health, biosensing has played an important role in monitoring contaminants and biomarkers. In the past decade, the integration of CRISPR-Cas systems with technologies like microfluidic devices and isothermal amplification methods has paved the way for developing advanced sensors for environmental surveillance. Here we discuss the recent progress of various CRISPR-Cas systems to develop new biosensing devices, ranging from the fundamental mechanisms to their practical applications. We present a comprehensive and critical overview on the current state-of-the-art of CRISPR-Cas-based sensing platforms, including for both nucleic acid and non-nucleic acid contaminants, as well as portable engineered systems for on-site detection. We also provide the prospects of CRISPR-Cas systems for next-generation environmental surveillance, together with emerging technologies such as data science and artificial intelligence.