Browsing by Author "Bouras, Noureddine"
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Item Open Access Biological activities of Streptomyces sp. Bts40 isolated from the rhizosphere of Artemisia herba-alba Asso(Universitatea din Oradea, 2022-01-04) Djemouai, Nadjette; Meklat, Atika; Gaceb-Terrak, Rabéa; Oulad Hadj Youcef, Khadidja; Nacer, Asma; Mokrane, Salim; Bouras, Noureddine; Verheecke-Vaessen, CarolActinobacteria isolated from the rhizosphere of plants are of interest as they produce a diverse range of molecules, such as antibiotics and enzymes. This study investigates the antibacterial activity, plant growth-promoting (PGP) abilities as well as the production of extracellular enzymes by the actinobacterial strain BTS40. This strain was isolated from the rhizospheric soil of the medicinal plant Artemisia herba-alba Asso that was naturally grown in a semi-arid environment. Morphological characteristics showed that the strain BTS40 belongs to the genus Streptomyces. Analysis of BTS40’s 16S rRNA gene sequence showed 99.45% similarity to Streptomyces alboniger NRRL B-1832T, in the EzTaxon database. This actinobacterium showed only antibacterial activity against Gram-positive bacteria. The strain also showed potential multiple traits for plant growth promotion and hydrolysis of enzymes. Hence, this study reveals that strain BTS40 has multiple PGP traits and produces many extracellular hydrolytic enzymes.Item Open Access Taxonomy of mycelial actinobacteria isolated from Saharan soils and their efficiency to reduce aflatoxin B1 content in a solid-based medium(Elsevier, 2017-02-02) Lahoum, Abdelhadi; Verheecke-Vaessen, Carol; Bouras, Noureddine; Sabaou, Nasserdine; Mathieu, FlorenceAflatoxin B1 (AFB1) is a carcinogenic compound produced by filamentous fungi. In order to reduce AFB1 occurrence in foodstuffs, 13 strains of mycelial actinobacteria were tested in vitro for the efficacy to reduce AFB1 content; all were isolated from the Saharan soils of Algeria. Firstly, morphological study and molecular analysis, based on the 16S rRNA gene, indicated that these strains belong to Actinomadura, Nocardiopsis, Nonomuraea, Saccharothrix and Streptomyces genera. Secondly, each strain’s efficacy to reduce pure AFB1 content was studied in ISP2-medium. After a 4-day incubation at 30°C on AFB1-supplemented medium (5 ppm of AFB1), AFB1 was extracted and quantified. AFB1 content was reduced by all strains (42.9–97.6%). The three most efficient reducers (94.9–97.6%) were two strains belonging to the genus Streptomyces and one to the genus Saccharothrix. Among the latter, strains ACD6 and ABH19 showed no adsorption mechanism involved, suggesting a potential degradation mechanism. These findings led us to suggest that these actinobacterial strains could be used as decontamination treatments for the reduction of AFB1 content.