Browsing by Author "Bitchagno, Gabin T. M."

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    The first comprehensive chemical profiling of Vachellia gummifera (Willd.) Kyal. & Boatwr., a plant with medicinal value
    (Wiley, 2024-06-01) Kisiriko, Musa; Noleto-Dias, Clarice; Bitchagno, Gabin T. M.; Naboulsi, Imane; Anastasiadi, Maria; Terry, Leon A.; Sobeh, Mansour; Beale, Michael H.; Ward, Jane L.
    Vachellia gummifera (Willd.) Kyal. & Boatwr. is a medicinal plant endemic to Morocco that has no documented studies on its chemical composition. In this study, the chemical composition of the water/methanol (4 : 1) extracts of air-dried leaf and stem samples of Moroccan V. gummifera was determined using UHPLC-MS and NMR. In total, over 100 metabolites were identified in our study. Pinitol was the major compound in both the leaf and stem extracts, being significantly more abundant in the former. Asparagine and 3-hydroxyheteroendrin were the second most abundant compounds in the stem and leaf extracts, respectively, though both compounds were present in each tissue. The other compounds included flavonoids based on quercetin, and phenolic derivatives. Eucomic acid, only identified in the stems and was the major aromatic compound distinguishing the leaf and stem profiles. Quercetin 3-O-(6′′-O-malonyl)-β-D-glucopyranoside was identified as the major flavonoid in the leaves but was also present in the stems. Other malonylated derivatives that were all flavonol glycosides based on myricetin, kaempferol, and isorhamnetin in addition to quercetin were also identified. This is the first report of eucomic acid and malonylated compounds in Vachellia species. This report provides valuable insights into the chemotaxonomic significance of the Vachellia genus.
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    The untargeted metabolomic analysis of Ammodaucus leucotrichus Coss. & Dur. seeds reveals previously undescribed polar lignans and terpenoids
    (Elsevier, 2025-02-01) Kisiriko, Musa; Bitchagno, Gabin T. M.; Noleto-Dias, Clarice; Naboulsi, Imane; Anastasiadi, Maria; Terry, Leon A.; Sobeh, Mansour; Beale, Michael H.; Ward, Jane L.
    The polar metabolome of the medicinal plant Ammodaucus leucotrichus Coss. & Dur. has not been comprehensively characterised. In this study, the chemical composition of a water/methanol (4:1) extract of seeds was determined through a combination of UHPLC-MS and NMR techniques. Sixty compounds were identified from the extract with 36 of these confirmed using UHPLC-MS and/or NMR data while the remaining 24 were given putative identifications based on UHPLC-MS data. The compounds included lignans, terpenes, phenolics, flavonoids, and alkaloid derivatives together with some amino- and organic acids. Of these 2 terpenoids and 3 lignans were found to be novel and were isolated and structures determined by comprehensive NMR studies. A further novel lignan glycoside was tentatively identified. Together these data represent the most comprehensive profile of this traditional medicinal plant, providing an annotated profile that can form the basis of future correlative metabolomic investigations to determine active principles behind its reported bioactivities.