Browsing by Author "Bhatia, Shashi Kant"
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Item Open Access Bread waste – a potential feedstock for sustainable circular biorefineries(Elsevier, 2022-12-21) Kumar, Vinod; Brancoli, Pedro; Narisetty, Vivek; Wallace, Stephen; Charalampopoulos, Dimitris; Kumar Dubey, Brajesh; Kumar, Gopalakrishnan; Bhatnagar, Amit; Bhatia, Shashi Kant; Taherzadeh, Mohammad J.The management of staggering volume of food waste generated (∼1.3 billion tons) is a serious challenge. The readily available untapped food waste can be promising feedstock for setting up biorefineries and one good example is bread waste (BW). The current review emphasis on capability of BW as feedstock for sustainable production of platform and commercially important chemicals. It describes the availability of BW (>100 million tons) to serve as a feedstock for sustainable biorefineries followed by examples of platform chemicals which have been produced using BW including ethanol, lactic acid, succinic acid and 2,3-butanediol through biological route. The BW-based production of these metabolites is compared against 1G and 2G (lignocellulosic biomass) feedstocks. The review also discusses logistic and supply chain challenges associated with use of BW as feedstock. Towards the end, it is concluded with a discussion on life cycle analysis of BW-based production and comparison with other feedstocks.Item Open Access Detection of bacterial pathogens and antibiotic residues in chicken meat: a review(MDPI, 2020-10-20) Kumar, Harsh; Bhardwaj, Kanchan; Kaur, Talwinder; Nepovimova, Eugenie; Kuča, Kamil; Kumar, Vinod; Bhatia, Shashi Kant; Dhanjal, Daljeet Singh; Chopra, Chirag; Singh, Reena; Guleria, Shivani; Bhalla, Tek Chand; Verma, Rachna; Kumar, DineshDetection of pathogenic microbes as well as antibiotic residues in food animals, especially in chicken, has become a matter of food security worldwide. The association of various pathogenic bacteria in different diseases and selective pressure induced by accumulated antibiotic residue to develop antibiotic resistance is also emerging as the threat to human health. These challenges have made the containment of pathogenic bacteria and early detection of antibiotic residue highly crucial for robust and precise detection. However, the traditional culture-based approaches are well-comprehended for identifying microbes. Nevertheless, because they are inadequate, time-consuming and laborious, these conventional methods are not predominantly used. Therefore, it has become essential to explore alternatives for the easy and robust detection of pathogenic microbes and antibiotic residue in the food source. Presently, different monitoring, as well as detection techniques like PCR-based, assay (nucleic acid)-based, enzyme-linked immunosorbent assays (ELISA)-based, aptamer-based, biosensor-based, matrix-assisted laser desorption/ionization-time of flight mass spectrometry-based and electronic nose-based methods, have been developed for detecting the presence of bacterial contaminants and antibiotic residues. The current review intends to summarize the different techniques and underline the potential of every method used for the detection of bacterial pathogens and antibiotic residue in chicken meat.Item Open Access Enhanced biohydrogen generation through calcium peroxide engendered efficient ultrasonic disintegration of waste activated sludge in low temperature environment(Elsevier, 2022-10-27) Banu, J. Rajesh; Preethi; Gunasekaran, M.; Kumar, Vinod; Bhatia, Shashi Kant; Kumar, GopalakrishnanWaste activated sludge is a renewable source for biohydrogen production, whereas the presence of complex biopolymers limits the hydrolysis step during this process, and thus pretreatment is required to disintegrate the sludge biomass. In this study, the feasibility of utilizing waste activated sludge to produce biohydrogen by improving the solubilization by means of thermo CaO2 engendered sonication disintegration (TCP-US) was studied. The optimized condition for extracellular polymeric substance (EPS) dissociation was obtained at the CaO2 dosage of 0.05 g/g SS at 70 °C. The maximum disintegration after EPS removal was achieved at the sonic specific energy input of 1612.8 kJ/kg TS with the maximum solubilization and SS reduction of 23.7% and 18.14%, respectively, which was higher than the US alone pretreatment. Thus, this solubilization yields higher biohydrogen production of 114.3 mLH2/gCOD in TCP-US sample.Item Open Access Fermentative production of 2,3-Butanediol using bread waste – A green approach for sustainable management of food waste(Elsevier, 2022-06-01) Narisetty, Vivek; Zhang, Le; Zhang, Jingxin; Lin, Carol Sze Ki; Tong, Yen Wah; Show, Pau Loke; Bhatia, Shashi Kant; Misra, Ashish; Kumar, VinodBread is Europe’s most wasted food, and the second most wasted food after potatoes in UK. Bread waste (BW) is a clean source of high-quality fermentable sugars. In this study, the potential of Enterobacter ludwigii to accumulate 2,3-butanediol (BDO) from BW was evaluated. Initially, the optimal inoculum size and yeast extract concentration were determined, followed by extraction of sugars from BW using acid and enzymatic hydrolysis. A glucose yield of 330–530 g/kg BW was obtained, and the sugars released were utilised for BDO production by E. ludwigii. The fed-batch cultivation using pure glucose and glucose rich hydrolysates from acid and enzymatic hydrolysis resulted in BDO titres of 144.5, 135.4, and 138.8 g/L, after 96 h, with yield of 0.47, 0.42 and 0.48 g/g yield, respectively. The innovation of the work is valorisation of BW to BDO with a circular biorefining approach and thus, reducing BW disposal and associated environmental burden.Item Open Access From brew to table: unleashing the potential of spent grains protein(Elsevier, 2024-03-27) Ahuja, Vishal; Chauhan, Shikha; Yang, Yung-Hun; Bhatia, Shashi Kant; Kumar, VinodUnited Nations and Upcycled Food Association have suggested the possible use of food processing residues and waste materials to improve food quality as well as overcome hunger and malnutrition. Brewer's spent grains (BSG) account 85% of brewery waste having high nutritive value. Spent grains are initially exploited for animal feed only and restricted to the local area due to high moisture content that leads to spoilage. Spent grains are rich in fibre and proteins and were explored for the preparation of cookies, bread, pasta, and noodles which showed higher nutritive value and health benefits in comparison to conventional materials. The bioactive compounds in spent grains also provide additional advantages and can be used in drug formulation for commercial products. However, it needs in-depth research and technical support that aid in contributing to the circular economy.Item Open Access Fruit extract mediated green synthesis of metallic nanoparticles: a new avenue in pomology applications(MDPI, 2020-11-11) Kumar, Harsh; Bhardwaj, Kanchan; Dhanjal, Daljeet Singh; Nepovimova, Eugenie; Șen, Fatih; Regassa, Hailemeleak; Singh, Reena; Verma, Rachna; Kumar, Vinod; Kumar, Dinesh; Bhatia, Shashi Kant; Kuča, KamilFruit extracts have natural bioactive molecules that are known to possess significant therapeutic potential. Traditionally, metallic nanoparticles were synthesized via chemical methods, in which the chemical act as the reducing agent. Later, these traditional metallic nanoparticles emerged as the biological risk, which prompted researchers to explore an eco-friendly approach. There are different eco-friendly methods employed for synthesizing these metallic nanoparticles via the usage of microbes and plants, primarily via fruit extract. These explorations have paved the way for using fruit extracts for developing nanoparticles, as they eliminate the usage of reducing and stabilizing agents. Metallic nanoparticles have gained significant attention, and are used for diverse biological applications. The present review discusses the potential activities of phytochemicals, and it intends to summarize the different metallic nanoparticles synthesized using fruit extracts and their associated pharmacological activities like anti-cancerous, antimicrobial, antioxidant and catalytic efficiencyItem Open Access Microbial exopolysaccharide composites in biomedicine and healthcare: trends and advances(MDPI, 2023-04-06) Ahuja, Vishal; Bhatt, Arvind Kumar; Banu, J. Rajesh; Kumar, Vinod; Kumar, Gopalakrishnan; Yang, Yung-Hun; Bhatia, Shashi KantMicrobial exopolysaccharides (EPSs), e.g., xanthan, dextran, gellan, curdlan, etc., have significant applications in several industries (pharma, food, textiles, petroleum, etc.) due to their biocompatibility, nontoxicity, and functional characteristics. However, biodegradability, poor cell adhesion, mineralization, and lower enzyme activity are some other factors that might hinder commercial applications in healthcare practices. Some EPSs lack biological activities that make them prone to degradation in ex vivo, as well as in vivo environments. The blending of EPSs with other natural and synthetic polymers can improve the structural, functional, and physiological characteristics, and make the composites suitable for a diverse range of applications. In comparison to EPS, composites have more mechanical strength, porosity, and stress-bearing capacity, along with a higher cell adhesion rate, and mineralization that is required for tissue engineering. Composites have a better possibility for biomedical and healthcare applications and are used for 2D and 3D scaffold fabrication, drug carrying and delivery, wound healing, tissue regeneration, and engineering. However, the commercialization of these products still needs in-depth research, considering commercial aspects such as stability within ex vivo and in vivo environments, the presence of biological fluids and enzymes, degradation profile, and interaction within living systems. The opportunities and potential applications are diverse, but more elaborative research is needed to address the challenges. In the current article, efforts have been made to summarize the recent advancements in applications of exopolysaccharide composites with natural and synthetic components, with special consideration of pharma and healthcare applications.Item Open Access Molecular biology interventions for activity improvement and production of industrial enzymes(Elsevier, 2020-12-24) Bhatia, Shashi Kant; Vivek, Narisetty; Kumar, Vinod; Chandel, Neha; Thakur, Meenu; Kumar, Dinesh; Yang, Yung-Hun; Pugazhendhi, Arivalagan; Kumar, GopalakrishnanMetagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display, etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is a need to overcome limitations of maintaining activity during the catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewedItem Open Access Progress in microalgal mediated bioremediation systems for the removal of antibiotics and pharmaceuticals from wastewater(Elsevier, 2022-02-16) Chandel, Neha; Ahuja, Vishal; Gurav, Ranjit; Kumar, Vinod; Tyagi, Vinay Kumar; Pugazhendhi, Arivalagan; Kumar, Gopalakrishnan; Kumar, Deepak; Yang, Yung-Hun; Bhatia, Shashi KantWorldwide demand for antibiotics and pharmaceutical products is continuously increasing for the control of disease and improvement of human health. Poor management and partial metabolism of these compounds result in the pollution of aquatic systems, leading to hazardous effects on flora, fauna, and ecosystems. In the past decade, the importance of microalgae in micropollutant removal has been widely reported. Microalgal systems are advantageous as their cultivation does not require additional nutrients: they can recover resources from wastewater and degrade antibiotics and pharmaceutical pollutants simultaneously. Bioadsorption, degradation, and accumulation are the main mechanisms involved in pollutant removal by microalgae. Integration of microalgae-mediated pollutant removal with other technologies, such as biodiesel, biochemical, and bioelectricity production, can make this technology more economical and efficient. This article summarizes the current scenario of antibiotic and pharmaceutical removal from wastewater using microalgae-mediated technologies.Item Open Access Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry(Springer Nature, 2024-05-29) Kumar, Vinod; Kumar, Pankaj; Maity, Sunil K.; Agrawal, Deepti; Narisetty, Vivek; Jacob, Samuel; Kumar, Gopalakrishnan; Bhatia, Shashi Kant; Kumar, Dinesh; Vivekanand, VivekanandSuccinic acid (SA) is one of the top platform chemicals with huge applications in diverse sectors. The presence of two carboxylic acid groups on the terminal carbon atoms makes SA a highly functional molecule that can be derivatized into a wide range of products. The biological route for SA production is a cleaner, greener, and promising technological option with huge potential to sequester the potent greenhouse gas, carbon dioxide. The recycling of renewable carbon of biomass (an indirect form of CO2), along with fixing CO2 in the form of SA, offers a carbon-negative SA manufacturing route to reduce atmospheric CO2 load. These attractive attributes compel a paradigm shift from fossil-based to microbial SA manufacturing, as evidenced by several commercial-scale bio-SA production in the last decade. The current review article scrutinizes the existing knowledge and covers SA production by the most efficient SA producers, including several bacteria and yeast strains. The review starts with the biochemistry of the major pathways accumulating SA as an end product. It discusses the SA production from a variety of pure and crude renewable sources by native as well as engineered strains with details of pathway/metabolic, evolutionary, and process engineering approaches for enhancing TYP (titer, yield, and productivity) metrics. The review is then extended to recent progress on separation technologies to recover SA from fermentation broth. Thereafter, SA derivatization opportunities via chemo-catalysis are discussed for various high-value products, which are only a few steps away. The last two sections are devoted to the current scenario of industrial production of bio-SA and associated challenges, along with the author's perspective.Item Open Access A review on the pollution assessment of hazardous materials and the resultant biorefinery products in Palm oil mill effluent(Elsevier, 2023-04-24) Meena, Anu Alias; Merrylin, J.; Banu, Rajesh; Bhatia, Shashi Kant; Kumar, Vinod; Piechota, Grzegorz; Kumar, GopalakrishnanThe voluminous nature of POME is directly associated with environmental hazards and could be turned into biorefinery products. The POME, rich in BOD, COD, and oil and grease, with few hazardous materials such as siloxanes, fatty acid methyl ester, and phenolic compounds may significantly increase the risk of violating the effluent quality standards. Recently, the application of chemical and biological risk assessment that can use electrochemical sensors and microalgae-like species has gained paramount attention towards its remediation. This review describes the existing risk assessment for POME and recommends a novel assessment approach using fish species including invasive ones as suitable for identifying the toxicants. Various physico-chemical and biological treatments such as adsorption, coagulation-flocculation, photo-oxidation, solar-assisted extraction, anaerobic digestion, integrated anaerobic-aerobic, and microalgae cultivation has been investigated. This paper offers an overview of anaerobic technologies, with particular emphasis on advanced bioreactors and their prospects for industrial-level applications. To illustrate, palmitic acid and oleic acid, the precursors of fatty acid methyl ester found in POME pave the way to produce biodiesel with 91.45%. Although there are some challenges in attaining production at an economic scale, this review offers some opportunities that could help in overcoming these challenges.Item Open Access Sustainable utilization and valorization of potato waste: state of the art, challenges, and perspectives(Springer, 2023-07-26) Khanal, Sonali; Karimi, Keikhosro; Majumdar, Sneha; Kumar, Vinod; Verma, Rachna; Bhatia, Shashi Kant; Kuca, Kamil; Esteban, Jesús; Kumar, DineshPotatoes (Solanum tuberosum L.) are starchy tuberous crops that represent the world’s fourth most important crop. According to the “Food and Agricultural Organisation of the United Nations,” 376 million metric tonnes of potatoes were produced globally in 2021. It is anticipated that around 8000 kilotons of potato peel waste might be generated in 2030, with related greenhouse gas emissions of 5 million tonnes of CO2 equivalent. Waste generated throughout the food supply chain, from potato farms to forks, contributes considerably to global warming. Apart from animal feed, technical processes in the potato processing industry generate waste organic residues with high amounts of bioactive compounds and carbohydrates, and thus extraction and bioconversion processes can produce high-value products. These include the isolation of functional ingredients for the formulation of nutraceuticals and pharma products, bioenergy-related products, enzymes, and fertilizers for the market, hence contributing to a more responsible production and consumption and, overall, circular economy. This review focuses on studies involving potato waste from industries such as pulp, processed water, peels, and mash showing the great prospects of the by-products from this crop for value-added supply chains and the reduction of undesired environmental effects. This work attempts to adhere upon several Sustainable Development Goals, specifically goals 7, 12, and 13, while also contributing to long-term growth targets and reducing negative environmental consequences produced by the food sector.Item Open Access Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries(Royal Society of Chemistry, 2021-10-26) Narisetty, Vivek; Cox, Rylan; Bommareddy, Rajesh; Agrawal, Deepti; Ahmad, Ejaz; Pant, Kamal Kumar; Chandel, Anuj Kumar; Bhatia, Shashi Kant; Kumar, Dinesh; Binod, Parmeswaran; Gupta, Vijai Kumar; Kumar, VinodBiologists and engineers are making tremendous efforts in contributing to a sustainable and green society. To that end, there is growing interest in waste management and valorisation. Lignocellulosic biomass (LCB) is the most abundant material on the earth and an inevitable waste predominantly originating from agricultural residues, forest biomass and municipal solid waste streams. LCB serves as the renewable feedstock for clean and sustainable processes and products with low carbon emission. Cellulose and hemicellulose constitute the polymeric structure of LCB, which on depolymerisation liberates oligomeric or monomeric glucose and xylose, respectively. The preferential utilization of glucose and/or absence of the xylose metabolic pathway in microbial systems cause xylose valorization to be alienated and abandoned, a major bottleneck in the commercial viability of LCB-based biorefineries. Xylose is the second most abundant sugar in LCB, but a non-conventional industrial substrate unlike glucose. The current review seeks to summarize the recent developments in the biological conversion of xylose into a myriad of sustainable products and associated challenges. The review discusses the microbiology, genetics, and biochemistry of xylose metabolism with hurdles requiring debottlenecking for efficient xylose assimilation. It further describes the product formation by microbial cell factories which can assimilate xylose naturally and rewiring of metabolic networks to ameliorate xylose-based bioproduction in native as well as non-native strains. The review also includes a case study that provides an argument on a suitable pathway for optimal cell growth and succinic acid (SA) production from xylose through elementary flux mode analysis. Finally, a product portfolio from xylose bioconversion has been evaluated along with significant developments made through enzyme, metabolic and process engineering approaches, to maximize the product titers and yield, eventually empowering LCB-based biorefineries. Towards the end, the review is wrapped up with current challenges, concluding remarks, and prospects with an argument for intense future research into xylose-based biorefineries.