Browsing by Author "Bashkatov, A. N."
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Item Open Access The Enhancement of Confocal Images Of Tissues at Bulk Optical Immersion.(Springer Science Business Media, 2003-01-01T00:00:00Z) Meglinski, I. V.; Bashkatov, A. N.; Genina, E. A.; Churmakov, D. Y.; Tuchin, V. V.The purpose of the present work is a theoretical examination of how localised skin-tissue dehydration affects the depth of the confocal probing and what depth of effective detection can be reached with the chemical administration of skin tissues. A semi-infinite multilayer Monte Carlo model is used to estimate spatial localisation of the output signal offered by a confocal probe. A solution of glycerol is taken in the capacity of innocuous osmotic agent. Diffusion of this bio-compatible chemical agent into the skin temporarily pushes water out of the tissues and results in the matching of the refractive indices of skin structural elements. This temporarily decreases scattering and increases transparency of topical skin layers, which allows for unrestricted light to permeate deeper into the skin. The results of simulation show that signal spatial localization offered by a confocal probe in the skin tissues during their clearing is usable for the monitoring of deep reticular dermis and improving the image contrast and spatial resolution. A discussion of the optical properties of skin tissues and their changes due to diffusion of glycerol into the skin is given. Optical properties of tissues and their changes due to chemical administration are estimated based on the results of experimental in vitro study with rat and human skin.Item Open Access Study of the possibility of increasing the probing depth by the method of reflection confocal microscopy upon immersion clearing of near-surface human skin layers.(Turpion Ltd., 2002-01-01T00:00:00Z) Meglinski, I. V.; Bashkatov, A. N.; Genina, E. A.; Churmakov, D. Y.; Tuchin, V. V.The possibility of increasing the human-skin probing depth by the method of reflection confocal microscopy (RCM) upon decreasing the amplitude of spatial fluctuations of the refractive index of the upper skin layers is considered. A change in the probing depth is estimated by analysing the spatial distribution of the probability density of the effective optical paths of detected photons calculated by the Monte Carlo method. The results of the numerical simulation are interpreted within the framework of the possible application of RCM to the study of the human skin exposed to an immersion liquid compatible to it. A diffusion of the immersion agent into the skin depth involves the equalising of the refractive indices of the structural elements of near-surface skin layers, which in turn causes a decrease in the scattering intensity and a certain increase in the transparency of the upper tissue layers. It is shown that a decrease in the light scattering in the near-surface skin layers leads to a significant increase in the probing depth obtained with the RCM technique.