Staff publications - Cranfield University at Silsoe

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Browsing Staff publications - Cranfield University at Silsoe by Author "Barton, Andrew C."

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    Labeless AC impedimetric antibody-based sensors with pg ml-1 sensitivities for point-of-care biomedical applications.
    (Elsevier Science B.V., Amsterdam., 2009-01-01T00:00:00Z) Barton, Andrew C.; Collyer, Stuart D.; Davis, Frank; Garifallou, Goulielmos-Zois; Tsekenis, Georgios; Tully, Elizabeth; O'Kennedy, Richard; Gibson, Tim; Millner, Paul A.; Higson, Seamus P. J.
    This paper describes the development and characterisation of labeless immunosensors for (a) the cardiac drug digoxin and (b) bovine serum albumin (BSA). Commercial screen-printed carbon electrodes were used as the basis for the sensors. Two methods were used to immobilise antibodies at the electrode surface. Aniline was electropolymerised onto these electrodes to form a thin planar film of conductive polyaniline; the polyaniline film was then utilised as a substrate to immobilise biotinylated anti-digoxin using a classical avidin- biotin affinity approach. As an alternative approach, poly(1,2-diaminobenzene) was electrodeposited onto the carbon electrodes and this modified surface was then sonochemically ablated to form an array of micropores. A second electropolymerisation step was then used to co-deposit conductive polyaniline along with antibodies for BSA within these pores to produce a microarray of polyaniline protrusions with diameters of several μm, containing entrapped anti- BSA
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    Sonochemically fabricated microelectrode arrays for biosensors offering widespread applicability. Part I
    (Elsevier Science B.V., Amsterdam., 2004-09-15T00:00:00Z) Barton, Andrew C.; Collyer, Stuart D.; Davis, Frank; Gornall, Davinia D.; Law, Karen A.; Lawrence, Emma C. D.; Mills, Daniel W.; Myler, Suzy; Pritchard, Jeanette A.; Thompson, Mark; Higson, Seamus P. J.
    A novel and patented procedure is described for the sonochemical fabrication of a new class of microelectrode array based sensor with electrode element populations of up to 2 x 105 cm-2. For some years it has been accepted that microelectrode arrays offer an attractive route for lowering minimum limits of detection and imparting stir (convectional mass transport) independence to sensor responses; despite this no commercial biosensors, to date, have employed microelectrode arrays, largely due to the cost of conventional fabrication routes that have not proved commercially viable for disposable devices. Biosensors formed by our sonochemical approach offer unrivalled sensitivity and impart stir independence to sensor responses. This format lends itself for mass fabrication due to the simplicity and inexpensiveness of the approach; in the first instance impedimetric and amperometric sensors are reported for glucose as model systems. Sensors already developed for ethanol, oxalate and a number of pesticide determinations will be reported in subsequent publications.