Baral, PratibhaPundir, AnushkaKurmi, AkhileshSingh, RaghuvirKumar, VinodAgrawal, Deepti2021-05-122021-05-122021-04-18Baral P, Pundi A, Kumar A, et al., (2021) Salting-out assisted solvent extraction of L (+) lactic acid obtained after fermentation of sugarcane bagasse hydrolysate. Separation and Purification Technology, Volume 269, August 2021, Article number 1187881383-5866https://doi.org/10.1016/j.seppur.2021.118788http://dspace.lib.cranfield.ac.uk/handle/1826/16670Lactic acid is among the twelve platform chemicals produced from inexpensive and renewable feedstocks such as lignocellulosic biomass. The present study illustrates salting-out assisted solvent extraction of L (+) lactic acid, derived from sugarcane bagasse hydrolysate. Screening studies with various extractants and diluents revealed that a combination of tri-n-butyl phosphate and ethyl acetate yielded the best results and extracted 59.63 ± 1.28% lactic acid from the dilute fermentation broth adjusted to a pH of 1.6 ± 0.2. Various inorganic salts were screened to enhance extraction efficiency further. The addition of 60% (w/v) ammonium sulfate improved the lactic acid extraction by 36.17%. This salt concentration successfully extracted 85.95 ± 0.44% lactic acid to the organic phase under optimized conditions from the fermentation broth at a pH of ~ 2.5 containing 40–100 g L−1 lactic acid. Recovery of > 80% salt is also shown using chilled acetone, which upon reuse showed a nominal decline of 3.3% during extraction. Thus an eco-friendly approach using a green solvent like ethyl acetate with mild operating conditions is demonstrated in the present study.enAttribution-NonCommercial-NoDerivatives 4.0 InternationalLactic acidSugarcane bagasse hydrolysateExtractionAmmonium sulphateEthyl acetateTri-n-butyl phosphateArticle