Jobling, KellyQuintela-Baluja, MarcosHassard, FrancisAdamou, PanagiotaBlackburn, AdrianTerm Research TeamMcIntyre-Nolan, ShannonO'Mara, OscarRomalde, Jesus L.Di Cesare, MariachiaraGraham, David W.2024-01-182024-01-182023-12-02Jobling K, Quintela-Baluja M, Hassard F, et al., (2024) Comparison of gene targets and sampling regimes for SARS-CoV-2 quantification for wastewater epidemiology in UK prisons. Journal of Water and Health, Volume 22, Issue 1, January 2024, pp. 64-761477-8920https://doi.org/10.2166/wh.2023.093https://dspace.lib.cranfield.ac.uk/handle/1826/20682Prisons are high-risk settings for infectious disease transmission, due to their enclosed and semi-enclosed environments. The proximity between prisoners and staff, and the diversity of prisons reduces the effectiveness of non-pharmaceutical interventions, such as social distancing. Therefore, alternative health monitoring methods, such as wastewater-based epidemiology (WBE), are needed to track pathogens, including SARS-CoV-2. This pilot study assessed WBE to quantify SARS-CoV-2 prevalence in prison wastewater to determine its utility within a health protection system for residents. The study analysed 266 samples from six prisons in England over a 12-week period for nucleoprotein 1 (N1 gene) and envelope protein (E gene) using quantitative reverse transcriptase-polymerase chain reaction. Both gene assays successfully detected SARS-CoV-2 fragments in wastewater samples, with both genes significantly correlating with COVID-19 case numbers across the prisons (p < 0.01). However, in 25% of the SARS-positive samples, only one gene target was detected, suggesting that both genes be used to reduce false-negative results. No significant differences were observed between 14- and 2-h composite samples, although 2-h samples showed greater signal variance. Population normalisation did not improve correlations between the N1 and E genes and COVID-19 case data. Overall, WBE shows considerable promise for health protection in prison settings.enAttribution 4.0 InternationalCOVID-19health monitoringprisonsRT-qPCRSARS-CoV-2wastewater-based epidemiologyArticle1996-7829