Amraoui, YassinNarisetty, VivekCoulon, FredericAgrawal, DeeptiChandel, Anuj KumarMaina, SofiaKoutinas, ApostolisKumar, Vinod2021-07-212021-07-212021-07-16Amraoui Y, Narisetty V, Coulon F, et al., (2021) Integrated fermentative production and downstream processing of 2,3-butanediol from sugarcane bagasse-derived xylose by mutant strain of Enterobacter ludwigii. ACS Sustainable Chemistry and Engineering, Volume 9, Issue 30, August 2021, pp. 10381-103912168-0485https://doi.org/10.1021/acssuschemeng.1c03951http://dspace.lib.cranfield.ac.uk/handle/1826/16909In this study, a mutant strain of Enterobacter ludwigii developed in our previous work, was evaluated to utilize pure xylose as the sole carbon and energy source for 2,3-butanediol (BDO) production. Later, this strain was also investigated on detoxified and nondetoxified xylose-rich hydrolysate obtained from hydrothermally pretreated sugarcane bagasse (SCB) for BDO production. Supplementing the fermentation medium with 0.2% w/v yeast extract improved cell growth (31%), BDO titer (43%), and yield (41%) against the synthetic medium devoid of any complex nitrogen source. The fed-batch culture with cyclic control of pH resulted in a BDO production of 71.1 g/L from pure xylose with overall yield and productivity of 0.40 g/g and 0.94 g/L·h, respectively. While BDO titer, yield, and productivity of 63.5 g/L, 0.36 g/g, and 0.84 g/L·h, were acheived with detoxified hydrolysate, respectively. In contrast, 32.7 g/L BDO was produced from nondetoxified hydrolysate with a conversion yield of 0.33 g/g and a productivity of 0.43 g/L·h. BDO accumulated on pure xylose and detoxified SCB hydrolysate was separated by aqueous two-phase system (ATPS) method using (NH4)2SO4 as salting-out agent and isopropanol as an extractant, resulting in the BDO recovery of more than 85%. The results achieved in the current work exemplify a step toward industrial BDO production from cost-effective hemicellulosic hydrolysates by E. ludwigii.enAttribution-NonCommercial 4.0 Internationalaqueous two-phase system (ATPS) extraction2,3-butanediolEnterobacter ludwigiinondetoxified hydrolysatedetoxified hydrolysatexyloseArticle