Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers

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dc.contributor.authorGałecki, Maciej
dc.contributor.authorTartas, Adrianna
dc.contributor.authorSzymanek, Agata
dc.contributor.authorSims, Emma
dc.contributor.authorLundholm, Lovisa
dc.contributor.authorSollazzo, Alice
dc.contributor.authorCheng, Lei
dc.contributor.authorFujishima, Yohei
dc.contributor.authorYoshida, Mitsuaki A.
dc.contributor.authorŻygierewicz, Jarosław
dc.contributor.authorWojcik, Andrzej
dc.contributor.authorBrzozowska-Wardecka, Beata
dc.date.accessioned2019-09-03T13:42:08Z
dc.date.available2019-09-03T13:42:08Z
dc.date.issued2019-05-29
dc.description.abstractPurpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used when fast analysis of a large amount of data is needed. The aim of this study was to compare the performance of two radiobiological assays – chromosomal aberrations and micronuclei – by unexperienced scorers with the reference values generated by an expert. Materials and methods: Each participant of an EU-funded two-week radiobiology course was asked to score Chinese hamster ovary cells exposed to gamma radiation up to 4 Gy. The congruence of students’ and expert’s scores at each dose and the coherence of the dose-response curve parameters between the students were investigated. Results: Micronucleus test tended to be faster and easier to learn than scoring chromosomal aberrations. However, both assays carried out by inexperienced students showed reasonable dose-response curves. Conclusions: In the case of a large radiological accident involving many casualties, the unexperienced scorers would support the process of biodosimetric triage by cytogenetic biological dosimetry.en_UK
dc.identifier.citationGałecki M, Tartas A, Szymanek A, et al., (2019) Precision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorers. International Journal of Radiation Biology, Volume 95, Issue 9, June 2019, pp. 1251-1258en_UK
dc.identifier.issn0955-3002
dc.identifier.urihttps://doi.org/10.1080/09553002.2019.1625462
dc.identifier.urihttp://dspace.lib.cranfield.ac.uk/handle/1826/14504
dc.language.isoenen_UK
dc.publisherTaylor and Francisen_UK
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectChromosomal aberrationsen_UK
dc.subjectmicronucleien_UK
dc.subjectionizing radiationen_UK
dc.subjectradiobiological assaysen_UK
dc.titlePrecision of scoring radiation-induced chromosomal aberrations and micronuclei by unexperienced scorersen_UK
dc.typeArticleen_UK

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