dc.contributor.author |
Mustafa, M. K. |
|
dc.contributor.author |
Nabok, Alexei |
|
dc.contributor.author |
Parkinson, D. |
|
dc.contributor.author |
Tothill, Ibtisam E. |
|
dc.contributor.author |
Salam, Faridah |
|
dc.contributor.author |
Tsargorodskaya, A. |
|
dc.date.accessioned |
2011-01-24T15:03:57Z |
|
dc.date.available |
2011-01-24T15:03:57Z |
|
dc.date.issued |
2010-12-15 |
|
dc.identifier.citation |
M.K. Mustafa, A. Nabok, D. Parkinson, I.E. Tothill, F. Salam and A. Tsargorodskaya, Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics, Biosensors and Bioelectronics, Volume 26, Issue 4, 15 December 2010, Pages 1332-1336 |
en_UK |
dc.identifier.issn |
0956-5663 |
|
dc.identifier.uri |
http://dx.doi.org/10.1016/j.bios.2010.07.042 |
|
dc.identifier.uri |
http://dspace.lib.cranfield.ac.uk/handle/1826/4760 |
|
dc.description.abstract |
A highly sensitive method of spectroscopic ellipsometry in total internal reflection mode (TIRE) was exploited for detecting β-amyloid peptide (Aβ1–16) in the direct immune reaction with monoclonal DE2 antibodies (raised against Aβ1–16) electrostatically immobilised on the surface of gold. A rapid detection of Aβ1–16 in a wide range of concentrations from 5 μg/ml down to 0.05 ng/ml was achieved using a cost-effective and label-free direct immunoassay format. TIRE dynamic spectral measurements proved that the immune reaction between DE2 monoclonal antibodies and Aβ1–16 is highly specific with the affinity constant KD = 1.46 × 10−8 mol/l. The same DE2 antibodies were utilised for detection of amyloid precursor protein APP770, a larger protein containing Aβ1–16 domain, using the quartz crystal microbalance (QCM) measurements in liquid. A combination of QCM and TIRE kinetics results allowed the evaluation of the originally unknown concentration of APP770 in complete medium solution containing other proteins, salts, and amino acids. |
en_UK |
dc.language.iso |
en |
en_UK |
dc.publisher |
Elsevier |
en_UK |
dc.subject |
Alzheimer's disease |
en_UK |
dc.subject |
β-Amyloid peptide 1–16 |
en_UK |
dc.subject |
Amyloid precursor protein (APP770) |
en_UK |
dc.subject |
Direct immunoassay |
en_UK |
dc.subject |
Total internal reflection ellipsometry |
en_UK |
dc.subject |
Quartz crystal microbalance |
en_UK |
dc.title |
Detection of β-amyloid peptide (1–16) and amyloid precursor protein (APP770) using spectroscopic ellipsometry and QCM techniques: A step forward towards Alzheimers disease diagnostics |
en_UK |
dc.type |
Article |
en_UK |