The biochemistry and physiology of poly-beta-hydroxybutyrate metabolism in methylosinus trichosporium OB3b

dc.contributor.advisor	Best, D. J.
dc.contributor.author	Williams, A. M.
dc.date.accessioned	2010-06-19T15:18:26Z
dc.date.available	2010-06-19T15:18:26Z
dc.date.issued	1988-09
dc.identifier.uri	http://hdl.handle.net/1826/4449
dc.description.abstract	The obligate methanotroph, Methylosinus trichosporium 0B3b synthesised up to 20 % of its dry weight as poly-beta-hydroxybutyrate when grown in batch culture or nitrogen limited continuous culture on a methanol/ammonium salts medium. Four soluble enzymes associated with polymer metabolism, D(-)-3-hydroxybutyrate dehydrogenase (3-HBD), acetoacetyl-CoA synthetase, beta-ketothiolase and acetoacetyl-CoA reductase have been purified from the crude extract of this organism. Detailed kinetic studies were conducted on each enzyme and their role in the regulation of polymer metabolism was assessed. Cont/d.	en_UK
dc.language.iso	en	en_UK
dc.publisher	Cranfield University	en_UK
dc.title	The biochemistry and physiology of poly-beta-hydroxybutyrate metabolism in methylosinus trichosporium OB3b	en_UK
dc.type	Thesis or dissertation	en_UK
dc.type.qualificationlevel	Doctoral	en_UK
dc.type.qualificationname	PhD	en_UK