Physicochemical factors influence the abundance and culturability of human enteric pathogens and fecal indicator organisms in estuarine water and sediment

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dc.contributor.author Hassard, Francis
dc.contributor.author Andrews, Anthony
dc.contributor.author Jones, Davey L.
dc.contributor.author Parsons, Louise
dc.contributor.author Jones, Vera
dc.contributor.author Cox, Brian A.
dc.contributor.author Daldorph, Peter
dc.contributor.author Brett, Howard
dc.contributor.author McDonald, James E.
dc.contributor.author Malham, Shelagh K.
dc.date.accessioned 2017-10-19T09:14:20Z
dc.date.available 2017-10-19T09:14:20Z
dc.date.issued 2017-10-17
dc.identifier.citation Hassard F, Andrews A, Jones DL, Parsons L, Jones V, Cox BA, Daldorph P, Brett H, McDonald JE, Malham SK, Physicochemical factors influence the abundance and culturability of human enteric pathogens and fecal indicator organisms in estuarine water and sediment, Frontiers in Microbiology, Vol. 8, 17 October 2017, Article 1996, pp.1-18 en_UK
dc.identifier.issn 1664-302X
dc.identifier.uri http://dx.doi.org/10.3389/fmicb.2017.01996
dc.identifier.uri http://dspace.lib.cranfield.ac.uk/handle/1826/12661
dc.description.abstract To assess fecal pollution in coastal waters, current monitoring is reliant on culture-based enumeration of bacterial indicators, which does not account for the presence of viable but non-culturable or sediment-associated micro-organisms, preventing effective quantitative microbial risk assessment (QMRA). Seasonal variability in viable but non-culturable or sediment-associated bacteria challenge the use of fecal indicator organisms (FIOs) for water monitoring. We evaluated seasonal changes in FIOs and human enteric pathogen abundance in water and sediments from the Ribble and Conwy estuaries in the UK. Sediments possessed greater bacterial abundance than the overlying water column, however, key pathogenic species (Shigella spp., Campylobacter jejuni, Salmonella spp., hepatitis A virus, hepatitis E virus and norovirus GI and GII) were not detected in sediments. Salmonella was detected in low levels in the Conwy water in spring/summer and norovirus GII was detected in the Ribble water in winter. The abundance of E. coli and Enterococcus spp. quantified by culture-based methods, rarely matched the abundance of these species when measured by qPCR. The discrepancy between these methods was greatest in winter at both estuaries, due to low CFU's, coupled with higher gene copies (GC). Temperature accounted for 60% the variability in bacterial abundance in water in autumn, whilst in winter salinity explained 15% of the variance. Relationships between bacterial indicators/pathogens and physicochemical variables were inconsistent in sediments, no single indicator adequately described occurrence of all bacterial indicators/pathogens. However, important variables included grain size, porosity, clay content and concentrations of Zn, K, and Al. Sediments with greater organic matter content and lower porosity harbored a greater proportion of non-culturable bacteria (including dead cells and extracellular DNA) in winter. Here, we show the link between physicochemical variables and season which govern culturability of human enteric pathogens and FIOs. Therefore, knowledge of these factors is critical for accurate microbial risk assessment. Future water quality management strategies could be improved through monitoring sediment-associated bacteria and non-culturable bacteria. This could facilitate source apportionment of human enteric pathogens and FIOs and direct remedial action to improve water quality. en_UK
dc.language.iso en en_UK
dc.publisher Frontiers Media en_UK
dc.rights Attribution 4.0 International (CC BY 4.0) You are free to: Share — copy and redistribute the material in any medium or format Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Information: No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
dc.subject Sediment en_UK
dc.subject Fecal indicator organisms en_UK
dc.subject Pathogen en_UK
dc.subject Elemental analysis en_UK
dc.subject Viable but non-culturable bacteria en_UK
dc.title Physicochemical factors influence the abundance and culturability of human enteric pathogens and fecal indicator organisms in estuarine water and sediment en_UK
dc.type Article en_UK


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