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Browsing by Author "Whitcombe, Michael J."

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Introducing MINA-The Molecularly Imprinted Nanoparticles Assay

Shutov, Roman V.; Guerreiro, Antonio R.; Moczko, Ewa; Perez De Vargas Sansalvador, Isabel M.; Chianella, Iva; Whitcombe, Michael J.; Piletsky, Sergey A. (Wiley, 2014-02-06)

A new ELISA‐ (enzyme‐linked immunosorbent assay)‐like assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic ...
New reactive polymer for protein immobilisation on sensor surfaces

Kyprianou, Dimitris; Guerreiro, Antonio R.; Chianella, Iva; Piletska, Elena V.; Fowler, Steven A.; Karim, Kal; Whitcombe, Michael J.; Turner, Anthony P. F.; Piletsky, Sergey A. (Elsevier Science B.V., Amsterdam., 2009-01-01)

Immobilisation of biorecognition elements on transducer surfaces is a key step in the development of biosensors. The immobilisation needs to be fast, cheap and most importantly should not affect the biorecognition activity ...
Novel linear polymers able to inhibit bacterial quorum sensing

Cavaleiro, Eliana; Duarte, Ana Sofia; Esteves, Ana Cristina; Correia, António; Whitcombe, Michael J.; Piletska, Elena V.; Piletsky, Sergey A.; Chianella, Iva (Wiley, 2015-01-28)

Bacterial phenotypes, such as biofilm formation, antibiotic resistance and virulence expression, are associated with quorum sensing. Quorum sensing is a density-dependent regulatory system of gene expression controlled by ...
Patterned gallium surfaces as molecular mirrors

Bossi, Alessandra; Rivetti, Claudio; Mangiarotti, Laura; Whitcombe, Michael J.; Turner, Anthony P. F.; Piletsky, Sergey A. (Elsevier Science B.V., Amsterdam., 2007-09-30)

An entirely new means of printing molecular information on a planar film, involving casting nanoscale impressions of the template protein molecules in molten gallium, is presented here for the first time. The metallic ...
The rational development of molecularly imprinted polymer-based sensors for protein detection.

Whitcombe, Michael J.; Chianella, Iva; Larcombe, Lee; Piletsky, Sergey A.; Noble, James; Porter, Robert; Horgan, Adrian (Royal Society of Chemistry, 2011-02-22)

The detection of specific proteins as biomarkers of disease, health status, environmental monitoring, food quality, control of fermenters and civil defence purposes means that biosensors for these targets will become ...
Selection of imprinted nanoparticles by affinity chromatography

Guerreiro, Antonio R.; Chianella, Iva; Piletska, Elena V.; Whitcombe, Michael J.; Piletsky, Sergey A. (Elsevier Science B.V., Amsterdam., 2009-04-15)

Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography. Subsequent fractionation of these particles by affinity chromatography ...
The stabilisation of receptor structure in low cross-linked MIPs by an immobilised template

Garcinuno, Rosa M.; Chianella, Iva; Guerreiro, Antonio R.; Mijangos, Irene; Piletska, Elena V.; Whitcombe, Michael J.; Piletsky, Sergey A. (Royal Society of Chemistry, 2009-01-21)

In molecularly imprinted polymers (MIPs) a high level of cross-linking is usually important for preserving the receptor structure. We propose here an alternative approach for stabilising binding sites, which involves the ...
A sulfur-sulfur cross-linked polymer synthesized from a polymerizable dithiocarbamate as a source of dormant radicals

Garcia Con, Luis Miguel; Whitcombe, Michael J.; Piletska, Elena V.; Piletsky, Sergey A. (John Wiley & Sons, Ltd, 2010-06-01)
Synthesis of controlled polymeric cross-linked coatings via iniferter polymerisation in the presence of tetraethyl thiuram disulphide chain terminator

Bossi, Alessandra; Whitcombe, Michael J.; Uludag, Yildiz; Fowler, S.; Chianella, Iva; Subrahmanyam, S.; Sanchez, I.; Piletsky, Sergey A. (Elsevier Science B.V., Amsterdam., 2010-05-15)

A “grafting from” approach has been used for controlled deposition of cross- linked polymers by living radical polymerisation. Borosilicate glass was modified with N,N-diethylaminodithiocarbamoylpropyl(trimethoxy)silane, ...
The use of a quartz crystal microbalance as an analytical tool to monitor particle/surface and particle/particle interactions under dry ambient and pressurized conditions: a study using common inhaler components

Turner, Nicholas W.; Bloxham, M.; Piletsky, Sergey A.; Whitcombe, Michael J.; Chianella, Iva (Royal Society of Chemistry, 2016-11-24)

Metered dose inhalers (MDI) and multidose powder inhalers (MPDI) are commonly used for the treatment of chronic obstructive pulmonary diseases and asthma. Currently, analytical tools to monitor particle/particle and ...

Browsing by Author "Whitcombe, Michael J."

Browsing by Author "Whitcombe, Michael J."

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